Publications related to The Colored Revolution of Bioimaging

Comparison of Deconvolution Software: A User Point of View—Part 2

Deconvolution is an image processing technique that restores the effective object representation [3] [4], allowing to improve images analysis steps such as segmentation [1] or colocalization study [2]. We performed several deconvolution tests on different ...

EMS2010

Microfluidic devices made of UV-curable glue (NOA81) for fluorescence detection based applications

Nico de Rooij, Philip Wägli, Blaise Guélat

The UV-curable adhesive NOA81 (Norland Optical Adhesive) is a promising liquid photopolymer for low-cost microfluidic chip production. Different fabrication methods for NOA81 are suggested [1,2]. However, optical characterization for fluorescent based dete ...

2010

Comparison of Deconvolution Software in 3D Microscopy: A User Point of View—Part 1

Daniel Sage, Alessandra Griffa

Deconvolution is an image restoration technique which improves image contrast, resolution and signal to noise ratio. In modern optical microscopy and biological research deconvolution is becoming a fundamental processing step which allows for better image ...

EMS2010

Superresolution Imaging using Single-Molecule Localization

Suliana Manley

Superresolution imaging is a rapidly emerging new field of microscopy that dramatically improves the spatial resolution of light microscopy by over an order of magnitude (similar to 10-20-nm resolution), allowing biological processes to be described at the ...

2010

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Michaël Unser, Matthieu Guerquin-Kern, Ilker Bayram, Raquel Terrés Cristofani

Modern deconvolution algorithms are often specified as minimization problems involving a non-quadratic regularization functional. When the latter is a wavelet-domain

ℓ _{ 1 }

-norm that favors sparse solutions, the problem can be solved by a simple it ...

IEEE2010

How to obtain labeled proteins and what to do with them

Kai Johnsson, Marlon Hinner

We review new and established methods for the chemical modification of proteins in living cells and highlight recent applications. The review focuses on tag-mediated protein labeling methods, such as the tetracysteine tag and SNAP-tag, and new developments ...

Elsevier2010

Super-resolved position and orientation estimation of fluorescent dipoles using 3D steerable filters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolution by sequentially imaging sparse subsets of fluorophores, which are localized by means of Gaussian-based localization. Applied to fixed fluorophores, with dipole ...

2010

Super-resolved position and orientation estimation of fluorescent dipoles using 3-D steerable filters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

The diffraction patterns of fixed fluorophores are characteristic of the orientation of the molecules' underlying dipole. Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolution by sequentially imaging sparse subsets ...

Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2010

Digital holographic microscopy real-time monitoring of cytoarchitectural alterations during simulated microgravity

Christian Depeursinge, Christophe Pache, Jonas Kühn, Muhammed Fatih Toy, Jérôme Parent, Marcel Egli

Previous investigations on mammalian cells have shown that microgravity, either that experienced in space, or simulated on earth, causes severe cellular modifications that compromise tissue determination and function. The aim of this study is to investigat ...

2010

Targeted Photoswitchable Probe for Nanoscopy of Biological Structures

Theo Lasser, Kai Johnsson, Iwan Märki, Stefan Geissbühler, Noelia Laura Bocchio, Grazvydas Lukinavicius, Claudio Dellagiacoma, Sambashiva Banala

We introduce a photoswitchable O6-benzylguanine derivative and demonstrate its use for super-resolution microscopy of SNAP-tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP-tagged mi ...

Wiley-Blackwell2010

The Colored Revolution of Bioimaging

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Comparison of Deconvolution Software: A User Point of View—Part 2

Microfluidic devices made of UV-curable glue (NOA81) for fluorescence detection based applications

Comparison of Deconvolution Software in 3D Microscopy: A User Point of View—Part 1

Superresolution Imaging using Single-Molecule Localization

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

How to obtain labeled proteins and what to do with them

Super-resolved position and orientation estimation of fluorescent dipoles using 3D steerable filters

Super-resolved position and orientation estimation of fluorescent dipoles using 3-D steerable filters

Digital holographic microscopy real-time monitoring of cytoarchitectural alterations during simulated microgravity

Targeted Photoswitchable Probe for Nanoscopy of Biological Structures

Microfluidic devices made of UV-curable glue (NOA81) for fluorescence detection based applications

Comparison of Deconvolution Software in 3D Microscopy: A User Point of View—Part 1

Superresolution Imaging using Single-Molecule Localization

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Targeted Photoswitchable Probe for Nanoscopy of Biological Structures

How to obtain labeled proteins and what to do with them

Super-resolved position and orientation estimation of fluorescent dipoles using 3D steerable filters

Super-resolved position and orientation estimation of fluorescent dipoles using 3-D steerable filters

Comparison of Deconvolution Software: A User Point of View—Part 2

Digital holographic microscopy real-time monitoring of cytoarchitectural alterations during simulated microgravity