Transmission electron cryomicroscopyTransmission electron cryomicroscopy (CryoTEM), commonly known as cryo-EM, is a form of cryogenic electron microscopy, more specifically a type of transmission electron microscopy (TEM) where the sample is studied at cryogenic temperatures (generally liquid-nitrogen temperatures). Cryo-EM is gaining popularity in structural biology. The utility of transmission electron cryomicroscopy stems from the fact that it allows the observation of specimens that have not been stained or fixed in any way, showing them in their native environment.
Equirectangular projectionThe equirectangular projection (also called the equidistant cylindrical projection or la carte parallélogrammatique projection), and which includes the special case of the plate carrée projection (also called the geographic projection, lat/lon projection, or plane chart), is a simple map projection attributed to Marinus of Tyre, who Ptolemy claims invented the projection about AD 100. The projection maps meridians to vertical straight lines of constant spacing (for meridional intervals of constant spacing), and circles of latitude to horizontal straight lines of constant spacing (for constant intervals of parallels).
Scanning transmission electron microscopyA scanning transmission electron microscope (STEM) is a type of transmission electron microscope (TEM). Pronunciation is [stɛm] or [ɛsti:i:ɛm]. As with a conventional transmission electron microscope (CTEM), images are formed by electrons passing through a sufficiently thin specimen. However, unlike CTEM, in STEM the electron beam is focused to a fine spot (with the typical spot size 0.05 – 0.2 nm) which is then scanned over the sample in a raster illumination system constructed so that the sample is illuminated at each point with the beam parallel to the optical axis.
Probability distributionIn probability theory and statistics, a probability distribution is the mathematical function that gives the probabilities of occurrence of different possible outcomes for an experiment. It is a mathematical description of a random phenomenon in terms of its sample space and the probabilities of events (subsets of the sample space). For instance, if X is used to denote the outcome of a coin toss ("the experiment"), then the probability distribution of X would take the value 0.5 (1 in 2 or 1/2) for X = heads, and 0.
Projection (mathematics)In mathematics, a projection is an idempotent mapping of a set (or other mathematical structure) into a subset (or sub-structure). In this case, idempotent means that projecting twice is the same as projecting once. The restriction to a subspace of a projection is also called a projection, even if the idempotence property is lost. An everyday example of a projection is the casting of shadows onto a plane (sheet of paper): the projection of a point is its shadow on the sheet of paper, and the projection (shadow) of a point on the sheet of paper is that point itself (idempotency).
Random variableA random variable (also called random quantity, aleatory variable, or stochastic variable) is a mathematical formalization of a quantity or object which depends on random events. The term 'random variable' can be misleading as it is not actually random nor a variable, but rather it is a function from possible outcomes (e.g., the possible upper sides of a flipped coin such as heads and tails ) in a sample space (e.g., the set ) to a measurable space (e.g., in which 1 corresponding to and −1 corresponding to ), often to the real numbers.
Projection (linear algebra)In linear algebra and functional analysis, a projection is a linear transformation from a vector space to itself (an endomorphism) such that . That is, whenever is applied twice to any vector, it gives the same result as if it were applied once (i.e. is idempotent). It leaves its unchanged. This definition of "projection" formalizes and generalizes the idea of graphical projection. One can also consider the effect of a projection on a geometrical object by examining the effect of the projection on points in the object.
Orthographic map projectionOrthographic projection in cartography has been used since antiquity. Like the stereographic projection and gnomonic projection, orthographic projection is a perspective (or azimuthal) projection in which the sphere is projected onto a tangent plane or secant plane. The point of perspective for the orthographic projection is at infinite distance. It depicts a hemisphere of the globe as it appears from outer space, where the horizon is a great circle. The shapes and areas are distorted, particularly near the edges.
Conditional probability distributionIn probability theory and statistics, given two jointly distributed random variables and , the conditional probability distribution of given is the probability distribution of when is known to be a particular value; in some cases the conditional probabilities may be expressed as functions containing the unspecified value of as a parameter. When both and are categorical variables, a conditional probability table is typically used to represent the conditional probability.
Decision problemIn computability theory and computational complexity theory, a decision problem is a computational problem that can be posed as a yes–no question of the input values. An example of a decision problem is deciding by means of an algorithm whether a given natural number is prime. Another is the problem "given two numbers x and y, does x evenly divide y?". The answer is either 'yes' or 'no' depending upon the values of x and y. A method for solving a decision problem, given in the form of an algorithm, is called a decision procedure for that problem.
