Real-time dual-wavelength digital holographic microscopy for extended measurement range with enhanced axial resolution
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Life sciences have a constantly growing need for novel methodological approaches suitable to investigate the intracellular environment with increased temporal and spatial resolutions. Recently, optical near-field probes, such as laser-irradiated pointed me ...
The aim of the present PhD thesis is the elaboration of new, home-made Scanning Near-field Optical Microscope (SNOM or NSOM), and the demonstration of its great potential for high-resolution topography and fluorescence investigations of soft and solid samp ...
We report the fabrication, functionalization and testing of microdevices for cell culture and cell traction force measurements in three-dimensions (3D). The devices are composed of bent cantilevers patterned with cell-adhesive spots not lying on the same p ...
Optical Coherence Microscopy (OCM) is a three-dimensional imaging technique that provides cross-sectional views of the subsurface microstructure of biological tissue, with a high axial and lateral resolution. In OCM, a low time coherence light source is sp ...
In this work we present a sizeable and reversible spectral tuning of the resonances of a two-dimensional photonic crystal nanocavity by exploiting the introduction of a sub-wavelength size glass tip. The comparison between experimental near-field data and ...
Dark-field illumination is known to enhance scattering contrast in optical microscopy. We combined this concept with Fourier domain optical coherence microscopy (OCM). The detection and illumination paths are decoupled, and only the scattered light origina ...
The Investigation of highly confined light fields created by different mechanisms, such as refraction, diffraction, and scattering, are the main subject of this thesis. We distinguish a specific scattering phenomenon of dielectric microspheres from general ...
In this thesis work, we extend digital holographic microscopy (DHM) capabilities and applications range by using from two to twenty different wavelength laser sources, either simultaneously or sequentially. As an overview, we present methods to increase th ...
The variety of microstructures that form at low solidification speed in peritectic alloys, bands, and islands, or even coupled (or cooperative) growth of the primary alpha and peritectic beta phases, have been previously explained by nucleation-growth mech ...
Reflection digital holographic microscopy (DHM) is a very powerful technique allowing measuring topography with a sub-nanometer axial resolution from a single hologram acquisition. But as most of interferometer methods, the vertical range is limited to hal ...