Publications related to Optical tomography by digital holographic microscopy

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Portable advanced off-axis camera for quantitative phase microscopy

Christian Depeursinge

We propose and experimentally demonstrate a device in which common-path interferometry combined with off-axis holographic geometry is used to realize a digital holographic camera which can be attached to the camera port of a conventional transmission micro ...

Spie-Int Soc Optical Engineering2014

Digital holographic microscopy: a quantitative label-free microscopy technique for phenotypic screening

Gerardo Turcatti, Benjamin Rappaz, Etienne Shaffer

Digital Holographic Microscopy (DHM) is a label-free imaging technique allowing visualization of transparent cells with classical imaging cell culture plates. The quantitative DHM phase contrast image provided is related both to the intracellular refractiv ...

2014

Off-axis digital holographic camera for quantitative phase microscopy

Christian Depeursinge, Christophe Moser, Frédéric Montfort, Zahra Monemhaghdoust

We propose and experimentally demonstrate a digital holographic camera which can be attached to the camera port of a conventional microscope for obtaining digital holograms in a self-reference configuration, under short coherence illumination and in a sing ...

Optical Soc Amer2014

Full field vertical scanning in short coherence digital holographic microscope

Christian Depeursinge, Christophe Moser, Etienne Cuche, Frédéric Montfort

In Digital holography Microscopes (DHM) implemented in the so-called "off axis" configuration, the object and reference wave fronts are not co-planar but form an angle of a few degrees. This results into two main drawbacks. First, the contrast of the inter ...

Optical Soc Amer2013

Automated segmentation of multiple red blood cells with digital holographic microscopy

Pierre Marquet, Daniel Boss

We present a method to automatically segment red blood cells (RBCs) visualized by digital holographic microscopy (DHM), which is based on the marker-controlled watershed algorithm. Quantitative phase images of RBCs can be obtained by using off-axis DHM alo ...

Spie-Soc Photo-Optical Instrumentation Engineers2013

Double-helix enhanced axial localization in STED nanoscopy

Demetri Psaltis, Andreas Vasdekis, Donald Benjaman Conkey, Grégoire Pierre Jean Laporte

Stimulated Emission Depletion (STED) microscopy enables subdiffraction resolution in the imaging plane. However, STED's lateral improvement in resolution is generally better than the enhancement in the axial direction. Here, we combine conventional STED su ...

Optical Society of America2013

High-resolution cell outline segmentation and tracking from phase-contrast microscopy images

Jean-Jacques Meister, Charles Brepsant, Mark Etienne Ambühl

Accurate extraction of cell outlines from microscopy images is essential for analysing the dynamics of migrating cells. Phase-contrast microscopy is one of the most common and convenient imaging modalities for observing cell motility because it does not re ...

2012