Optical tomography with digital holographic microscopy

Three-dimensional optical imaging by tomographic techniques is presented, especially with object rotation in a transmission configuration. In digital holographic microscopy, the complex field is measured, which permits either to study transparent objects with varying index of refraction or to compute diffraction tomography in which the phase is needed.

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Florian Charrière, Christian Depeursinge, Nicolas Pavillon
EOS, 2009
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https://infoscience.epfl.ch/record/147241?ln=en

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In mathematics, a complex number is an element of a number system that extends the real numbers with a specific element denoted i, called the imaginary unit and satisfying the equation

Optical coherence tomography (OCT) is an imaging technique that uses low-coherence light to capture micrometer-resolution, two- and three-dimensional images from within optical scattering media (e.g.,

Tomography is imaging by sections or sectioning that uses any kind of penetrating wave. The method is used in radiology, archaeology, biology, atmospheric science, geophysics, oceanography, plasma p

Related concepts

Related publications (7)

Related publications

Optical tomography by digital holographic microscopy

Florian Charrière, Christian Depeursinge, Jonas Kühn, Nicolas Pavillon

Optical tomography provides three-dimensional data of the measured specimen, while quantitative phase imaging enables measuring the induced phase-shifts. Combining those two technologies makes possible to get three-dimensional refractive index reconstruction. This can be achieved by introducing a scan in the measurement process, which can be done in several ways. We present and compare results of tomographic measurements, taken either in angle-scanning or wavelength-scanning approach, respectively in transmission or in reflection microscopy, in the framework of digital holographic microscopy.

SPIE2009

Time-domain optical coherence tomography with digital holographic microscopy

Florian Charrière, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Pia Massatsch

We show that digital holography can be combined easily with optical coherence tomography approach. Varying the reference path length is the means used to acquire a series of holograms at different depths, providing after reconstruction images of slices at different depths in the specimen thanks to the short- coherence length of light source. A metallic object, covered by a 150-µm-thick onion cell, is imaged with high resolution. Applications in ophthalmology are shown: structures of the anterior eye, the cornea, and the iris, are studied on enucleated porcine eyes. Tomographic images of the iris border close to the pupil were obtained 165 µm underneath the eye surface.

2005

Sub-cellular quantitative optical diffraction tomography with digital holographic microscopy

Florian Charrière, Tristan Colomb, Etienne Cuche, Christian Depeursinge, Jonas Kühn, Pierre Marquet

Digital holographic microscopy (DHM) is an interferometric technique, providing quantitative mapping of the phase shift induced by semi- transparent microscopic specimens, such as cells, with sub-wavelenght resolution along the optical axis. Thanks to actual PCs and CCDs, DHM provides nowadays cost-effective instruments for real-time measurements at very high acquisition rates, with sub-micron transverse resolution. However, DHM phase images do not reveal the three-dimensional (3D) internal distribution of refractive index, but a phase shift resulting from a mean refractive index (RI) integrated over the cellular thickness. Standard optical diffraction tomography (ODT) techniques can be efficiently applied to reveal internal structures and to measure 3D RI spatial distributions, by recording 2D DHM phase data for different sample orientations or illumination beam direction, in order to fill up entirely the Ewald sphere in the Fourier space. The 3D refractive index can then be reconstructed, even in the direct space with backpropagation algorithms or from the Fourier space with inverse Fourier transform. The presented technique opens wide perspectives in 3D cell imaging: the DHM-based micro-tomography furnishes invaluable data on the cell components optical properties, potentially leading to information about organelles intracellular distribution. Results obtained on biological specimens will be presented. Morphometric measurements can be extracted from the tomographic data, by detection based on the refractive index contrast within the 3D reconstructions. Results and perspectives about sub- cellular organelles identification inside the cell will also be exposed.

SPIE2007

In mathematics, a complex number is an element of a number system that extends the real numbers with a specific element denoted i, called the imaginary unit and satisfying the equation

Tomography is imaging by sections or sectioning that uses any kind of penetrating wave. The method is used in radiology, archaeology, biology, atmospheric science, geophysics, oceanography, plasma p