Three-dimensional nano-localization of single fluorescent emitters
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We demonstrate nanometer-level localization accuracy of a single fluorescent emitter in three dimensions. Our super resolution microscopy technique is based on spectral self-interference for axial localization and two-dimensional diffraction pattern analys ...
We show nanometer-level localization accuracy of a single quantum-dot in three dimensions by self-interference and diffraction-pattern analysis. We believe that this approach has the capacity to push optical microscopy to the molecular level. ...
The invention relates to large-field high-resolution microscopy and photolithography setups operating with polychromatic light. It includes the use of a plurality of focusing micromirrors. ...
We show nanometer-level localization accuracy of a single quantum-dot in three dimensions by self-interference and diffraction-pattern analysis. We believe that this approach has the capacity to push optical microscopy to the molecular level. ...
A new approach to quantitative single-molecule imaging by confocal laser scanning microscopy (CLSM) is presented. it relies on fluorescence intensity distribution to analyze the molecular occurrence statistics captured by digital imaging and enables direct ...
Biological microscopy favors photostable fluorescent markers with large fluorescence quantum yields, low dark triplet state population, good biocompatibility and absorption and emission maxima in the near-infrared, where cellular autofluorescence is minimi ...
We report on studies of Thermobifida fusca cellulases Cel5A, Cel6B and Cel9A binding to pretreated wood particles using Confocal Laser Scanning Microscopy (CLSM). Hydro-thermal pretreated wood particles were immobilized on borosilicate substrates before fl ...
The distributions of organic additives in self-leveling flooring compounds (SLCs) were investigated using laser-scanning microscopy for the selective visualization of the additives, which had been previously stained by a fluorescent dye. The distributions ...
Triplet, photo-oxidized and other photoinduced, long-lived states of fluorophores are sensitive to the local environment and thus attractive for microenvironmental imaging purposes. In this work, we introduce an approach where these states are monitored in ...
This thesis introduces a collection of physics-based methods for super-resolution in optical microscopy. The core of these methods constitutes a framework for 3-D localization of single fluorescent molecules. Localization is formulated as a parameter estim ...