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Harmonic holography: a new holographic principle

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Compact lensless digital holography

Manon Rostykus

Microscopy is of high interest for biology since it allows imaging features that are too small to be seen with naked eyes. However, cells are mostly transparent to visible and infrared light which makes it difficult to see with a traditional microscope. To ...

EPFL2018

Structural and functional imaging of cells, tissues and organisms is crucial for understanding biomedical processes. Fluorescence microscopy is an established tool and has contributed to many discoveries in the life sciences. This technique provides molecu ...

EPFL2017

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We propose and experimentally demonstrate a digital holographic camera which can be attached to the camera port of a conventional microscope for obtaining digital holograms in a self-reference configuration, under short coherence illumination and in a sing ...

Optical Soc Amer2014

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Digital Holographic Microscopy (DHM) is a label-free imaging technique allowing visualization of transparent cells with classical imaging cell culture plates. The quantitative DHM phase contrast image provided is related both to the intracellular refractiv ...

2014

High throughput second harmonic imaging for label-free biological applications

Pierre Magistretti, Sylvie Roke, Aleksandra Radenovic, Carlos Alberto Macias Romero, Orly Bagunu Tarun, Pierre Marquet, Pascal Jourdain, Vitalijs Zubkovs, Marie Eve Pascale Didier

Second harmonic generation (SHG) is inherently sensitive to the absence of spatial centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical changes and electrochemical responses. Here, we seek to improve the imaging th ...

Optical Society of America2014

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We present for the first time experimental measurements on the ultrafast population dynamics of the model surface-active dye methyl orange (MO) during its ion transfer across the water|1,2-dichloroethane interface by using time-resolved surface second harm ...

Amer Chemical Soc2014

Developing luminescent Brownian probes for near-field investigations of the intracellular environment

Flavio Maurizio Mor

Life sciences have a constantly growing need for novel methodological approaches suitable to investigate the intracellular environment with increased temporal and spatial resolutions. Recently, optical near-field probes, such as laser-irradiated pointed me ...

EPFL2013

Double-helix enhanced axial localization in STED nanoscopy

Demetri Psaltis, Andreas Vasdekis, Donald Benjaman Conkey, Grégoire Pierre Jean Laporte

Stimulated Emission Depletion (STED) microscopy enables subdiffraction resolution in the imaging plane. However, STED's lateral improvement in resolution is generally better than the enhancement in the axial direction. Here, we combine conventional STED su ...

Optical Society of America2013

Interest in high resolution imaging techniques has recently multiplied due to their importance in bio-medical research. Quantitative phase measurements by holographic microscopy is an extraordinary tool to gain new understanding of transparent biological s ...

EPFL2012

High-resolution cell outline segmentation and tracking from phase-contrast microscopy images

Jean-Jacques Meister, Charles Brepsant, Mark Etienne Ambühl

Accurate extraction of cell outlines from microscopy images is essential for analysing the dynamics of migrating cells. Phase-contrast microscopy is one of the most common and convenient imaging modalities for observing cell motility because it does not re ...

2012