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Harmonic holography: a new holographic principle

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Holographic second harmonic generation (SHG) microscopy

Christian Depeursinge, Pierre Marquet, Etienne Shaffer

The coherent nature of second harmonic generation (SHG) makes possible retrieval of both its amplitude and its phase, by use of a proper phase-sensitive imaging technique such as digital holographic SHG microscopy. In addition to retrieving both amplitude ...

2011

Novel Concepts for Functional High Resolution Microscopy

Matthias Geissbühler

Functional imaging of biological cells and organs is one of the key techniques that drives new discoveries in life science research and medicine. In this thesis novel concepts for functional imaging at high spatial and temporal resolution are presented. In ...

EPFL2011

Matching Pursuit Iterative Dipole Based Filter of Background Fields in Phase Imaging

Rolf Gruetter, Yves Wiaux, José Pedro Rebelo Ferreira Marques

The information present in the phase of gradient-echo images has opened a new window to look at fine brain anatomy. To obtain high quality phase images depicting small field perturbations produced by tissue susceptibility differences, large slowly spatiall ...

2011

Dark-field optical coherence microscopy

Theo Lasser, Christophe Pache, Martin Villiger

Dark-field illumination is known to enhance scattering contrast in optical microscopy. We combined this concept with Fourier domain optical coherence microscopy (OCM). The detection and illumination paths are decoupled, and only the scattered light origina ...

Optical Society of America2010

Real time, nanometric 3D-tracking of nanoparticles made possible by second harmonic generation digital holographic microscopy

Christian Depeursinge, Pierre Marquet, Etienne Shaffer

In the past decade, quantitative phase imaging gave a new dimension to optical microscopy, and the recent extension of digital holography techniques to nonlinear microscopy appears very promising, for the phase of nonlinear signal provides additional infor ...

Optical Society of America2010

Digital holography for second harmonic microscopy: application to 3D-tracking of nanoparticles

Christian Depeursinge, Etienne Shaffer

Retrieval of the amplitude and phase of electromagnetic waves made digital holographic microscopy (DHM) capable of revealing morphological details at ultrahigh resolution in the order of a few nanometers only and precisely measuring the refractive index ac ...

SPIE2010

Sub-Rayleigh resolution by phase imaging

Christian Depeursinge, Nicolas Pavillon, Etienne Shaffer, Muhammed Fatih Toy, Yann Jérôme Michel Pascal Cotte

We report the experimental observation of systematically occurring phase singularities in coherent imaging of sub-Rayleigh distanced objects. A theory that relates the observation to the sub-Rayleigh distance is presented and compared with experimental mea ...

Optical Society of America2010

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We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of ...

Optical Society of America2010

Enhanced Second Harmonic Generation in Plasmonic Nanocavities

Demetri Psaltis, Ye Pu, Rachel Grange, Chia-Lung Hsieh

We experimentally demonstrate significantly enhanced second harmonic generation using nanoengineered plasmonic nanocavities of core-shell structures (BaTiO3/Au). An enhancement factor of over 500 is measured in the second harmonic scattering efficiency com ...

Ieee Service Center, 445 Hoes Lane, Po Box 1331, Piscataway, Nj 08855-1331 Usa2010

Optical second harmonic generation, thanks to its coherent nature, is a suitable signal for interferometric measurements, such as digital holography: a well-established imaging technique that allows recovering of the complex diffraction wavefields from whi ...

SPIE2009