Nucleic acid structureNucleic acid structure refers to the structure of nucleic acids such as DNA and RNA. Chemically speaking, DNA and RNA are very similar. Nucleic acid structure is often divided into four different levels: primary, secondary, tertiary, and quaternary. Nucleic acid sequence Primary structure consists of a linear sequence of nucleotides that are linked together by phosphodiester bond. It is this linear sequence of nucleotides that make up the primary structure of DNA or RNA.
KnotA knot is an intentional complication in cordage which may be practical or decorative, or both. Practical knots are classified by function, including hitches, bends, loop knots, and splices: a hitch fastens a rope to another object; a bend fastens two ends of a rope to each another; a loop knot is any knot creating a loop; and splice denotes any multi-strand knot, including bends and loops. A knot may also refer, in the strictest sense, to a stopper or knob at the end of a rope to keep that end from slipping through a grommet or eye.
Nucleic acid double helixIn molecular biology, the term double helix refers to the structure formed by double-stranded molecules of nucleic acids such as DNA. The double helical structure of a nucleic acid complex arises as a consequence of its secondary structure, and is a fundamental component in determining its tertiary structure. The term entered popular culture with the publication in 1968 of The Double Helix: A Personal Account of the Discovery of the Structure of DNA by James Watson.
Knot (mathematics)In mathematics, a knot is an embedding of the circle S^1 into three-dimensional Euclidean space, R3 (also known as E3). Often two knots are considered equivalent if they are ambient isotopic, that is, if there exists a continuous deformation of R3 which takes one knot to the other. A crucial difference between the standard mathematical and conventional notions of a knot is that mathematical knots are closed — there are no ends to tie or untie on a mathematical knot.
Crossing number (knot theory)In the mathematical area of knot theory, the crossing number of a knot is the smallest number of crossings of any diagram of the knot. It is a knot invariant. By way of example, the unknot has crossing number zero, the trefoil knot three and the figure-eight knot four. There are no other knots with a crossing number this low, and just two knots have crossing number five, but the number of knots with a particular crossing number increases rapidly as the crossing number increases.
Ti plasmidA tumour inducing (Ti) plasmid is a plasmid found in pathogenic species of Agrobacterium, including [[Agrobacterium tumefaciens|A. tumefaciens]], A. rhizogenes, A. rubi and A. vitis. Evolutionarily, the Ti plasmid is part of a family of plasmids carried by many species of Alphaproteobacteria. Members of this plasmid family are defined by the presence of a conserved DNA region known as the repABC gene cassette, which mediates the replication of the plasmid, the partitioning of the plasmid into daughter cells during cell division as well as the maintenance of the plasmid at low copy numbers in a cell.
CohesinCohesin is a protein complex that mediates sister chromatid cohesion, homologous recombination, and DNA looping. Cohesin is formed of SMC3, SMC1, SCC1 and SCC3 (SA1 or SA2 in humans). Cohesin holds sister chromatids together after DNA replication until anaphase when removal of cohesin leads to separation of sister chromatids. The complex forms a ring-like structure and it is believed that sister chromatids are held together by entrapment inside the cohesin ring.
Trefoil knotIn knot theory, a branch of mathematics, the trefoil knot is the simplest example of a nontrivial knot. The trefoil can be obtained by joining together the two loose ends of a common overhand knot, resulting in a knotted loop. As the simplest knot, the trefoil is fundamental to the study of mathematical knot theory. The trefoil knot is named after the three-leaf clover (or trefoil) plant. The trefoil knot can be defined as the curve obtained from the following parametric equations: The (2,3)-torus knot is also a trefoil knot.
Linking numberIn mathematics, the linking number is a numerical invariant that describes the linking of two closed curves in three-dimensional space. Intuitively, the linking number represents the number of times that each curve winds around the other. In Euclidean space, the linking number is always an integer, but may be positive or negative depending on the orientation of the two curves (this is not true for curves in most 3-manifolds, where linking numbers can also be fractions or just not exist at all).
Molecular cloningMolecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA.
