Orthogonal frequency-division multiplexingIn telecommunications, orthogonal frequency-division multiplexing (OFDM) is a type of digital transmission used in digital modulation for encoding digital (binary) data on multiple carrier frequencies. OFDM has developed into a popular scheme for wideband digital communication, used in applications such as digital television and audio broadcasting, DSL internet access, wireless networks, power line networks, and 4G/5G mobile communications. OFDM is a frequency-division multiplexing (FDM) scheme that was introduced by Robert W.
Second-harmonic generationSecond-harmonic generation (SHG, also called frequency doubling) is a nonlinear optical process in which two photons with the same frequency interact with a nonlinear material, are "combined", and generate a new photon with twice the energy of the initial photons (equivalently, twice the frequency and half the wavelength), that conserves the coherence of the excitation. It is a special case of sum-frequency generation (2 photons), and more generally of harmonic generation.
CameraA camera is an optical instrument used to capture and store images or videos, either digitally via an electronic , or chemically via a light-sensitive material such as photographic film. As a pivotal technology in the fields of photography and videography, cameras have played a significant role in the progression of visual arts, media, entertainment, surveillance, and scientific research. The invention of the camera dates back to the 19th century and has since evolved with advancements in technology, leading to a vast array of types and models in the 21st century.
Fluorescence microscopeA fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to study the properties of organic or inorganic substances. "Fluorescence microscope" refers to any microscope that uses fluorescence to generate an image, whether it is a simple set up like an epifluorescence microscope or a more complicated design such as a confocal microscope, which uses optical sectioning to get better resolution of the fluorescence image.
Mirrorless cameraA mirrorless camera is a camera that does not have a mirror. Though most cameras, including smartphones, lack mirrors, the term mirrorless is primarily used to describe digital interchangeable lens cameras, in order to distinguish them from DSLRs, which have historically dominated interchangeable lens cameras. Lacking a mirror system allows the camera to be smaller, quieter, and lighter. In cameras with mirrors, light from the lens is directed to either the image sensor or the viewfinder.
Spatial frequencyIn mathematics, physics, and engineering, spatial frequency is a characteristic of any structure that is periodic across position in space. The spatial frequency is a measure of how often sinusoidal components (as determined by the Fourier transform) of the structure repeat per unit of distance. The SI unit of spatial frequency is cycles per meter (m). In applications, spatial frequency is often expressed in units of cycles per millimeter (mm) or equivalently per mm. In wave propagation, the spatial frequency is also known as wavenumber.
Two-photon excitation microscopyTwo-photon excitation microscopy (TPEF or 2PEF) is a fluorescence imaging technique that is particularly well-suited to image scattering living tissue of up to about one millimeter in thickness. Unlike traditional fluorescence microscopy, where the excitation wavelength is shorter than the emission wavelength, two-photon excitation requires simultaneous excitation by two photons with longer wavelength than the emitted light. The laser is focused onto a specific location in the tissue and scanned across the sample to sequentially produce the image.
Digital cameraA digital camera is a camera that captures photographs in digital memory. Most cameras produced today are digital, largely replacing those that capture images on photographic film. Digital cameras are now widely incorporated into mobile devices like smartphones with the same or more capabilities and features of dedicated cameras (which are still available). High-end, high-definition dedicated cameras are still commonly used by professionals and those who desire to take higher-quality photographs.
Movie cameraA movie camera (also known as a film camera and cine-camera) is a type of photographic camera that rapidly takes a sequence of photographs, either onto film stock or an , in order to produce a moving image to display on a screen. In contrast to the still camera, which captures a single image at a time, the movie camera takes a series of images by way of an intermittent mechanism or by electronic means; each image is a frame of film or video.
Depth of fieldThe depth of field (DOF) is the distance between the nearest and the furthest objects that are in acceptably sharp focus in an image captured with a camera. For cameras that can only focus on one object distance at a time, depth of field is the distance between the nearest and the farthest objects that are in acceptably sharp focus. "Acceptably sharp focus" is defined using a property called the "circle of confusion". The depth of field can be determined by focal length, distance to subject, the acceptable circle of confusion size, and aperture.
