Publications related to Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

Three-dimensional nano-localization of single fluorescent emitters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler, Noelia Laura Bocchio

We present a combination of self-interference microscopy with lateral super-resolution microscopy and introduce a novel approach for localizing a single nano-emitter to within a few nanometers in all three dimensions over a large axial range. We demonstrat ...

Optical Society of America2010

Scanning Near-field Optical Microscopy-based Study of Local Dynamics of Receptor-Ligand Interactions at the Single Molecule Level

Giovanni Dietler, Mounir Driss Mensi, Serguei Sekatski, Kanat Dukenbayev

A scanning near-field optical microscope (SNOM)-based modification of the method to study the dynamics of single molecule receptor?ligand interactions exploiting the fluorescence imaging by total internal reflection fluorescence microscopy is introduced. T ...

2010

Super-resolved position and orientation estimation of fluorescent dipoles using 3D steerable filters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolution by sequentially imaging sparse subsets of fluorophores, which are localized by means of Gaussian-based localization. Applied to fixed fluorophores, with dipole ...

2010

Microfluidic devices made of UV-curable glue (NOA81) for fluorescence detection based applications

Nico de Rooij, Philip Wägli, Blaise Guélat

The UV-curable adhesive NOA81 (Norland Optical Adhesive) is a promising liquid photopolymer for low-cost microfluidic chip production. Different fabrication methods for NOA81 are suggested [1,2]. However, optical characterization for fluorescent based dete ...

2010

Super-resolved position and orientation estimation of fluorescent dipoles using 3-D steerable filters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

The diffraction patterns of fixed fluorophores are characteristic of the orientation of the molecules' underlying dipole. Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolution by sequentially imaging sparse subsets ...

Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2010

Fluorescent nanopigments: Quantitative assessment of their quantum yield

Libero Zuppiroli, Rolando Ferrini

In the last few years, an intense research effort has focused on the synthesis of fluorescent nanopigments for functional inks, light harvesting, tagging, tracing, (bio)labeling, imaging, and lighting applications. Moreover, combined with dielectric matric ...

American Institute of Physics2010

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Michaël Unser, Matthieu Guerquin-Kern, Ilker Bayram, Raquel Terrés Cristofani

Modern deconvolution algorithms are often specified as minimization problems involving a non-quadratic regularization functional. When the latter is a wavelet-domain

ℓ _{ 1 }

-norm that favors sparse solutions, the problem can be solved by a simple it ...

IEEE2010

Turn-Off-and-On: Chemosensing Ensembles for Sensing Chloride in Water by Fluorescence Spectroscopy

Kay Severin, Kurt Schenk

The recognition and sensing of aqueous chloride by synthetic receptors is a challenging task. Herein we apply the chemosensing ensemble methodology to optically detect chloride in water at near physiological pH. Variants based on two closely related recept ...

2010

Cell Morphology and Intracellular Ionic Homeostasis explored with a Multimodal Approach combining Epifluorescence and Digital Holographic Microscopy

Pierre Magistretti, Christian Depeursinge, Nicolas Pavillon, Pierre Marquet, Jonas Kühn, Pascal Jourdain, Daniel Boss, Aleksandr Benke, Corinne Moratal

The authors have developed a live-cell multimodality microscope combining epifluorescence with digital holographic microscopy; it has been implemented with a decoupling procedure allowing to separately measure from the quantitative phase important cell par ...

2010

Cross-Reactive Sensor Arrays for the Detection of Peptides in Aqueous Solution by Fluorescence Spectroscopy

Kay Severin, Sébastien Rochat, Friederike Gundula Marie Zaubitzer, Jie Gao

A simple but powerful method for the sensing of peptides in aqueous solution has been developed. The transition-metal complexes [PdCl2(en)], [{RhCl2Cp*}2], and [{RuCl2(p-cymene)} 2] were combined with six different fluorescent dyes to build a cross-reactiv ...

2010

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

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Three-dimensional nano-localization of single fluorescent emitters

Scanning Near-field Optical Microscopy-based Study of Local Dynamics of Receptor-Ligand Interactions at the Single Molecule Level

Super-resolved position and orientation estimation of fluorescent dipoles using 3D steerable filters

Microfluidic devices made of UV-curable glue (NOA81) for fluorescence detection based applications

Super-resolved position and orientation estimation of fluorescent dipoles using 3-D steerable filters

Fluorescent nanopigments: Quantitative assessment of their quantum yield

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Turn-Off-and-On: Chemosensing Ensembles for Sensing Chloride in Water by Fluorescence Spectroscopy

Cell Morphology and Intracellular Ionic Homeostasis explored with a Multimodal Approach combining Epifluorescence and Digital Holographic Microscopy

Cross-Reactive Sensor Arrays for the Detection of Peptides in Aqueous Solution by Fluorescence Spectroscopy

Microfluidic devices made of UV-curable glue (NOA81) for fluorescence detection based applications

Super-resolved position and orientation estimation of fluorescent dipoles using 3D steerable filters

Three-dimensional nano-localization of single fluorescent emitters

Scanning Near-field Optical Microscopy-based Study of Local Dynamics of Receptor-Ligand Interactions at the Single Molecule Level

Super-resolved position and orientation estimation of fluorescent dipoles using 3-D steerable filters

Fluorescent nanopigments: Quantitative assessment of their quantum yield

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Cross-Reactive Sensor Arrays for the Detection of Peptides in Aqueous Solution by Fluorescence Spectroscopy

Turn-Off-and-On: Chemosensing Ensembles for Sensing Chloride in Water by Fluorescence Spectroscopy

Cell Morphology and Intracellular Ionic Homeostasis explored with a Multimodal Approach combining Epifluorescence and Digital Holographic Microscopy