Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI)
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Fluorescence confocal laser-scanning microscopy (LSM) is one of the most popular tools for life science research. This popularity is expected to grow thanks to single-photon array detectors tailored for LSM. These detectors offer unique single-photon spati ...
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited microscopy techniques and can image biological structures at the molecular scal ...
Significance: Fluorescence guidance is used clinically by surgeons to visualize anatomical and/or physiological phenomena in the surgical field that are difficult or impossible to detect by the naked eye. Such phenomena include tissue perfusion or molecula ...
Fluorescence microscopy is the method of choice to monitor dynamic processes in living cells due to its non-invasive nature. A variety of different fluorophores and labeling systems are currently used to selectively visualise structures or biomolecules of ...
Fluorescence super-resolution microscopy has allowed unprecedented insight into the workings of biological systems below the diffraction limit of light. Over the past decade, it has overcome several challenges to deliver 3D, multi-color and faster imaging ...
Optical microscopy is one widely used tool to study cell functions and the interaction of molecules at a sub-cellular level. Optical microscopy techniques can be broadly divided into two categories: partially coherent and incoherent. Coherent microscopy te ...
Fluorescence microscopy methods have been developed to circumvent the diffraction limit by exploiting nonlinearities in the interactions between light and fluorophores. Initially, these methods were up to orders of magnitude slower than standard microscopi ...
Studying dynamic biological processes, such as heart development and function in zebrafish embryos, often relies on multi-channel fluorescence labeling to distinguish multiple anatomical features, yet also demands high frame rates to capture rapid cell mot ...
Light microscopy is a tool of paramount importance for biologists and has been constantly improved for the past four centuries. Despite many recent developments, microscopy techniques still require improvement, especially to reach better temporal and spect ...
Overcoming the classical diffraction limit in optical microscopy is known to be achievable by a variety of far-field and near-field microscopy techniques. More recently, so-called micro-object-based optical super-resolution microscopy techniques have emerg ...