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Assessment of transferrin recycling by Triplet Lifetime Imaging in living cells

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Fluorometer system, fluorescene analysis method, and use thereof

Christoph Merten, Jatin Panwar

The invention relates to a fluorometer system for analyzing fluid samples comprising a plurality of particles capable of being labeled with at least two fluorescent labels, in particular two distinct fluorophores. The invention also relates to a fluorescen ...

2024

Networks of tryptophan (Trp)& horbar;an aromatic amino acid with strong fluorescence response & horbar;are ubiquitous in biological systems, forming diverse architectures in transmembrane proteins, cytoskeletal filaments, subneuronal elements, photorecepto ...

Amer Chemical Soc2024

[n]Cycloparaphenylenes as Compatible Fluorophores for Melt Electrowriting

Biranche Tandon

Fluorescent probes are an indispensable tool in the realm of bioimaging technologies, providing valuable insights into the assessment of biomaterial integrity and structural properties. However, incorporating fluorophores into scaffolds made from melt elec ...

Wiley-V C H Verlag Gmbh2024

A general strategy to develop fluorogenic polymethine dyes for bioimaging

Pablo Rivera Fuentes, Annabell Martin

Fluorescence imaging is an invaluable tool to study biological processes and further progress depends on the development of advanced fluorogenic probes that reach intracellular targets and label them with high specificity. Excellent fluorogenic rhodamine d ...

Berlin2023

Multivalent Peptide Ligands To Probe the Chromocenter Microenvironment in Living Cells

Beat Fierz, Pablo Rivera Fuentes, Nora Guidotti, Maxime Nicolas Mivelaz, Adam Miklos Eördögh

Chromatin is spatially organized into functional states that are defined by both the presence of specific histone post-translational modifications (PTMs) and a defined set of chromatin-associated “reader” proteins. Different models for the underlying mecha ...

2022

Single-photon avalanche diode imaging sensor for subsurface fluorescence LiDAR

Edoardo Charbon, Claudio Bruschini, Arin Can Ülkü

We realized subsurface fluorescence LiDAR with a large format single-photon avalanche diode array capable of localizing and quantifying concentration of fluorescent molecules in heavily scattering media, such as tissue, with submillimeter depth accuracy. ( ...

OPTICAL SOC AMER2021

A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy

Kai Johnsson, Lu Wang, Lin Xue

It is difficult to develop suitable fluorescent probes for live-cell nanoscopy, but a general strategy is now reported that can transform regular fluorophores into fluorogenic probes with excellent cell permeability and low unspecific background signals. U ...

NATURE PUBLISHING GROUP2020

Chemistry and Analysis of Nanoparticle-Modified Living Cells

Tanja Thomsen

Conventional therapeutics are often limited by their targeting ability, resulting in harmful and potential fatal side-effects for the patients. Recently, new strategies have been developed to improve target specificity of drugs in order to generate more ef ...

EPFL2020

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Fluorescence lifetime imaging (FLI) is increasingly recognized as a powerful tool for biochemical and cellular investigations, including in vivo applications. Fluorescence lifetime is an intrinsic characteristic of any fluorescent dye which, to a large ext ...

AMER CHEMICAL SOC2020

Fluorescence lifetime imaging with a megapixel SPAD camera and neural network lifetime estimation

Edoardo Charbon, Claudio Bruschini, Ming-Lo Wu, Kazuhiro Morimoto

Fluorescence lifetime imaging microscopy (FLIM) is a key technology that provides direct insight into cell metabolism, cell dynamics and protein activity. However, determining the lifetimes of different fluorescent proteins requires the detection of a rela ...

NATURE RESEARCH2020