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Digital holographic microscopy: a quantitative label-free microscopy technique for phenotypic screening

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NTIRE 2021 Depth Guided Image Relighting Challenge

Sabine Süsstrunk, Majed El Helou, Ruofan Zhou, Radu Timofte

Image relighting is attracting increasing interest due to its various applications. From a research perspective, image relighting can be exploited to conduct both image normalization for domain adaptation, and also for data augmentation. It also has multip ...

2021

Optical microscopy is one widely used tool to study cell functions and the interaction of molecules at a sub-cellular level. Optical microscopy techniques can be broadly divided into two categories: partially coherent and incoherent. Coherent microscopy te ...

EPFL2020

Microscopy is of high interest for biology since it allows imaging features that are too small to be seen with naked eyes. However, cells are mostly transparent to visible and infrared light which makes it difficult to see with a traditional microscope. To ...

EPFL2018

Coherent imaging from bacteria to multicellular organisms

Séverine Coquoz

Structural and functional imaging of cells, tissues and organisms is crucial for understanding biomedical processes. Fluorescence microscopy is an established tool and has contributed to many discoveries in the life sciences. This technique provides molecu ...

EPFL2017

Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy

Pierre Magistretti, Christian Depeursinge, Pierre Marquet, Pascal Jourdain, Daniel Boss

A dual-wavelength digital holographic microscope to measure absolute volume of living cells is proposed. The optical setup allows us to reconstruct two quantitative phase contrast images at two different wavelengths from a single hologram acquisition. When ...

Spie-Soc Photo-Optical Instrumentation Engineers2013

Exploring Neural Cell Dynamics with Digital Holographic Microscopy

Pierre Magistretti, Christian Depeursinge, Pierre Marquet

In this review, we summarize how the new concept of digital optics applied to the field of holographic microscopy has allowed the development of a reliable and flexible digital holographic quantitative phase microscopy (DH-QPM) technique at the nanoscale p ...

Annual Reviews2013

Resolution and Complex Deconvolution in Holographic Microscopy

Yann Jérôme Michel Pascal Cotte

Interest in high resolution imaging techniques has recently multiplied due to their importance in bio-medical research. Quantitative phase measurements by holographic microscopy is an extraordinary tool to gain new understanding of transparent biological s ...

EPFL2012

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Accurate extraction of cell outlines from microscopy images is essential for analysing the dynamics of migrating cells. Phase-contrast microscopy is one of the most common and convenient imaging modalities for observing cell motility because it does not re ...

2012

Early Cell Death Detection with Digital Holographic Microscopy

Pierre Magistretti, Christian Depeursinge, Nicolas Pavillon, Pierre Marquet, Pascal Jourdain, Corinne Moratal

Background: Digital holography provides a non-invasive measurement of the quantitative phase shifts induced by cells in culture, which can be related to cell volume changes. It has been shown previously that regulation of cell volume, in particular as it r ...

Public Library of Science2012

Beyond the lateral resolution limit by phase imaging

Christian Depeursinge, Muhammed Fatih Toy, Yann Jérôme Michel Pascal Cotte

We present a theory to extend the classical Abbe resolution limit by introducing a spatially varying phase into the illumination beam of a phase imaging system. It allows measuring lateral and axial distance differences between point sources to a higher ac ...

2011