Intensity and phase fields behind Phase Shifting Masks studied with High Resolution Interference Microscopy
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Optical microscopy is one widely used tool to study cell functions and the interaction of molecules at a sub-cellular level. Optical microscopy techniques can be broadly divided into two categories: partially coherent and incoherent. Coherent microscopy te ...
Structured light generation having broad applications in different optical fields, is the topic of this thesis. Our structured light generation strategy is based on applying periodic microoptical elements at the refraction-diffraction limit, under a focuse ...
Fluorescence super-resolution microscopy has allowed unprecedented insight into the workings of biological systems below the diffraction limit of light. Over the past decade, it has overcome several challenges to deliver 3D, multi-color and faster imaging ...
Overcoming the classical diffraction limit in optical microscopy is known to be achievable by a variety of far-field and near-field microscopy techniques. More recently, so-called micro-object-based optical super-resolution microscopy techniques have emerg ...
Well-established imaging techniques proved that features below the diffraction limit can be observed optically using so-called super-resolution microscopies, which overcome Abbe's resolution limit. In traditional far-field microscopy, the introduction of f ...
Microscopy is of high interest for biology since it allows imaging features that are too small to
be seen with naked eyes. However, cells are mostly transparent to visible and infrared light
which makes it difficult to see with a traditional microscope. To ...
Photolithography is one of the earliest technologies used to transfer patterns to a substrate. It is also known as optical lithography since it uses light to transfer the pattern. The main exposure techniques exist in the industry are projection printing, ...
Structural and functional imaging of cells, tissues and organisms is crucial for understanding biomedical processes. Fluorescence microscopy is an established tool and has contributed to many discoveries in the life sciences. This technique provides molecu ...
In this work, we apply the high-resolution interference microscopy technique to investigate the intensity and phase of light transmitted through a binary phase element. Using a combination of simulation and experimental results, we identify specific featur ...
The incorporation of hypericin (Hyp) from aqueous solutions into giant unilamellar vesicle (GUV) membranes has been studied experimentally and by means of kinetic Monte Carlo modeling. The time evolution of Hyp fluorescence originating from Hyp monomers di ...