Steerable Filters for Orientation Estimation and Localization of Fluorescent Dipoles
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Monitoring biological relevant reactions on the single molecule level based on fluorescence spectroscopy techniques has become one of the most promising approaches for understanding a variety of phenomena in biophysics, biochemistry and life science. By ap ...
With the recent development of fluorescent probes and new high-resolution microscopes, biological imaging has entered a new era and is presently having a profound impact on the way research is being conducted in the life sciences. Biologists have come to d ...
The past few years we have witnessed a tremendous surge of interest in so-called array-based miniaturised analytical systems due to their value as extremely powerful tools for high-throughput sequence analysis, drug discovery and development, and diagnosti ...
We introduce an efficient, image formation model-based algorithm that extends super-resolution fluorescence localization to include orientation estimation, and report experimental accuracies of 5 nanometers for position estimation and 2 degrees for dipole ...
Autofluorescence bronchoscopy is a promising approach to detect and characterize precancerous and early cancerous lesions. Nevertheless, many spectral features of photodetection systems remain unclear and sub-optimal at the present time. We report here a c ...
In this thesis a new approach for single molecule detection and analysis is explored. This approach is based on the combination of two well established methods, fluorescence correlation spectroscopy (FCS) and total internal reflection fluorescence microsco ...
In this thesis new imaging approaches for optical microscopy are proposed and studied. They are based on the use of dynamic structured illumination in combination with a demodulation detection concept employing CMOS image detectors. Two particular implemen ...
Single crystals of self-synthesized mordenite−Na were used for incorporation of the cationic dye molecule thionin blue (C12H10N3S+). The planar organic molecule (7.5 × 15 Å), which fits into the large 12-membered ring channel of mordenite, was incorporated ...