Site-Specific Glycoconjugation of Protein via Bioorthogonal Tetrazine Cycloaddition with a Genetically Encoded trans-Cyclooctene or Bicyclononyne
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We demonstrate the use of laser-induced. fluorescence confocal spectroscopy to measure analyte-stimulated enhanced green fluorescent protein (egfp) synthesis by genetically modified Escherichia coli bioreporter cells. Induction is measured in cell lysates ...
Studying proteins in living cells generally requires the construction of a fusion protein between the protein of interest and an appropriate tag. Currently used fusion protein-based assays rely on the genetically encoded properties of the tag, which signif ...
Fluorescent protein expressing cells with very high fluorescence are described as well as a method to obtain such cells. The fluorescence of such cells can be as high as about 1000 times the auto-fluorescence of the respective host cell. Due to the high fl ...
Mammalian cells in bioreactors as production host are the focus of this review. We wish to briefly describe today's technical status and to highlight emerging trends in the manufacture of recombinant therapeutic proteins, focusing on Chinese hamster ovary ...
This feature article provides a compilation of tools available for preparing well-defined peptide/protein–polymer conjugates, which are defined as hybrid constructs combining (i) a defined number of peptide/protein segments with uniform chain lengths and d ...
A fine control of a photonic molecule is obtained by nanofluidic techniques. The coupling condition between the modes of two photonic crystal nanocavities is modified by spectrally tuning each single resonator. Clear mode anticrossing and transition from l ...
We show that locally altering fused silica with femtosecond laser irradiation forms the basis for a novel manufacturing technology platform to produce highly integrated microsystems. In contrast to many common approaches that rely on combining materials to ...
This study is aimed at outlining the fabrication of a novel piezoresistive force sensor using LTCC technology, which operates at millinewton range, yet is compatible with low cost thick-film fabrication process. Mechanical and electrical characterization o ...
A review. Recombinant proteins are often expressed with an addnl. polypeptide the role of which is to aid purifn., detection, or functional studies. Recently, the role of these tags has been extended to mediate the labeling of the protein of interest with ...
A review of recent developments in the labeling of fusion proteins with synthetic mols. in the living cell or in complex mixts. The most commonly used tags are presented and then focuses on a new class of tags that are not limited to their genetically enco ...