Publications related to DeconvolutionLab2: An Open-Source Software for Deconvolution Microscopy

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Michaël Unser, Matthieu Guerquin-Kern, Ilker Bayram, Raquel Terrés Cristofani

Modern deconvolution algorithms are often specified as minimization problems involving a non-quadratic regularization functional. When the latter is a wavelet-domain

ℓ _{ 1 }

-norm that favors sparse solutions, the problem can be solved by a simple it ...

IEEE2010

Inline holographic coherent anti-Stokes Raman microscopy

Demetri Psaltis, Ho Ling Li, Kun Shi

We demonstrate a simple approach for inline holographic coherent anti-Stokes Raman scattering (CARS) microscopy, in which a layer of uniform nonlinear medium is placed in front of a specimen to be imaged. The reference wave created by four-wave mixing in t ...

2010

Fast and automated wavelet-regularized image restoration in fluorescence microscopy

Cédric René Jean Vonesch

The subject of this thesis is image restoration, that is, deconvolution and denoising. Our work is motivated by applications in the rapidly expanding field of biological imaging and in particular fluorescence microscopy. Inverse problems in this area typic ...

EPFL2009

Super-resolution fluorescence microscopy based on physical models

François Aguet

This thesis introduces a collection of physics-based methods for super-resolution in optical microscopy. The core of these methods constitutes a framework for 3-D localization of single fluorescent molecules. Localization is formulated as a parameter estim ...

EPFL2009

Optical tomography by digital holographic microscopy

Christian Depeursinge, Nicolas Pavillon, Jonas Kühn, Florian Charrière

Optical tomography provides three-dimensional data of the measured specimen, while quantitative phase imaging enables measuring the induced phase-shifts. Combining those two technologies makes possible to get three-dimensional refractive index reconstructi ...

SPIE2009

Single Molecule Interference: Towards Three-Dimensional Fluorescence Localization Microscopy at the Molecular Level

Theo Lasser, Iwan Märki

We demonstrate three-dimensional, nanometer-scale positioning precision of a single quantum-dot using single molecule interference and localization. We anticipate that our approach will lay the foundation for fluorescence microscopy with molecular resoluti ...

2009

A Fast Thresholded Landweber Algorithm for Wavelet-Regularized Multidimensional Deconvolution

Michaël Unser, Cédric René Jean Vonesch

We present a fast variational deconvolution algorithm that minimizes a quadratic data term subject to a regularization on the

\ell ^{ 1 }

-norm of the wavelet coefficients of the solution. Previously available methods have essentially consisted in alte ...

IEEE2008

An accurate PSF model with few parameters for axially shift-variant deconvolution

Michaël Unser, Dimitri Nestor Alice Van De Ville, François Aguet

Accurate knowledge of an imaging system's point spread function (PSF) is crucial for successful deconvolution. For fluorescence microscopy, PSF estimations based on either theoretical models or experimental measurements are available. However, due to the a ...

Ieee Service Center, 445 Hoes Lane, Po Box 1331, Piscataway, Nj 08855-1331 Usa2008

An Accurate PSF Model with Few Parameters for Axially Shift-Variant Deconvolution

Michaël Unser, Dimitri Nestor Alice Van De Ville, François Aguet

Accurate knowledge of an imaging system's point spread function (PSF) is crucial for successful deconvolution. For fluorescence microscopy, PSF estimations based on either theoretical models or experimental measurements are available. However, due to the ...

IEEE2008

A Fast Thresholded Landweber Algorithm for General Wavelet Bases: Application to 3D Deconvolution Microscopy

Michaël Unser, Cédric René Jean Vonesch

Wavelet-domain

ℓ _{ 1 }

-regularization is a promising approach to deconvolution. The corresponding variational problem can be solved using a “thresholded Landweber” (TL) algorithm. While this iterative procedure is simple to implement, it is known to ...

IEEE2008

DeconvolutionLab2: An Open-Source Software for Deconvolution Microscopy

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Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Inline holographic coherent anti-Stokes Raman microscopy

Fast and automated wavelet-regularized image restoration in fluorescence microscopy

Super-resolution fluorescence microscopy based on physical models

Optical tomography by digital holographic microscopy

Single Molecule Interference: Towards Three-Dimensional Fluorescence Localization Microscopy at the Molecular Level

A Fast Thresholded Landweber Algorithm for Wavelet-Regularized Multidimensional Deconvolution

An accurate PSF model with few parameters for axially shift-variant deconvolution

An Accurate PSF Model with Few Parameters for Axially Shift-Variant Deconvolution

A Fast Thresholded Landweber Algorithm for General Wavelet Bases: Application to 3D Deconvolution Microscopy

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Super-resolution fluorescence microscopy based on physical models

Inline holographic coherent anti-Stokes Raman microscopy

Fast and automated wavelet-regularized image restoration in fluorescence microscopy

Optical tomography by digital holographic microscopy

An accurate PSF model with few parameters for axially shift-variant deconvolution

Single Molecule Interference: Towards Three-Dimensional Fluorescence Localization Microscopy at the Molecular Level

An Accurate PSF Model with Few Parameters for Axially Shift-Variant Deconvolution

A Fast Thresholded Landweber Algorithm for Wavelet-Regularized Multidimensional Deconvolution

A Fast Thresholded Landweber Algorithm for General Wavelet Bases: Application to 3D Deconvolution Microscopy