Publication

Cellular Uptake and Intracellular Trafficking of Poly(N-(2-Hydroxypropyl) Methacrylamide)

Related publications (91)

Response characteristics of arsenic-sensitive bioreporters expressing the gfp reporter gene

This paper describes the development of an analytical technique for arsenic analyses that is based on genetically-modified bioreporter bacteria bearing a gene encoding for the production of a green fluorescent protein (gfp). Upon exposure to arsenic (in th ...

2005

Bottom-up approaches for organizing nanoparticles with polymers

Caterina Minelli

This thesis describes some of three years'work carried at the Centre Suisse d'Electronique et de Microtechnique (Neuchâtel, Switzerland) under the supervision of Dr. Martha Liley and in collaboration with Prof. Horst Vogel of the Ecole Polytechnique Fédéra ...

EPFL2004

Organization of nanoscale objects via polymer demixing

Horst Vogel, Harry Heinzelmann, Martha Liley, Caterina Minelli

We present an extremely versatile method for the lateral organization of nano-scale objects (NOs) based on the phenomenon of polymer demixing. NOs are suspended in a soln. of two immiscible polymers, which is used to form a thin polymer film by spin coatin ...

2004

Gold nano-antennas for increasing luminescence

Philippe Gillet

Energy transfer between nanoscale particles is demonstrated for a Gold/rare-earth oxide nanocomposite. The gold nanoparticle acts as an antenna, absorbing light that can then be transferred to the bound rare-earth oxide (see Figure), enhancing the oxide lu ...

2004

Fluorescent proteins in animal cells for process development: optimization of sodium butyrate treatment as an example

Florian Maria Wurm, Martin Jordan, Lisa Hunt

Fluorescent proteins expressed in mammalian cells can be quantified quickly and noninvasively with a standard fluorescence plate reader. We have previously exploited this quality in cell growth assessment (Hunt et al., 1999b). In this work, different CHO c ...

2002

Monitoring mis-acylated tRNA suppression efficiency in mammalian cells via EGFP fluorescence recovery

Horst Vogel, Horst Pick, Erwin Ilegems

A reporter assay was developed to detect and quantify nonsense codon suppression by chem. aminoacylated tRNAs in mammalian cells. It is based on the cellular expression of the enhanced green fluorescent protein (EGFP) as a reporter for the site-specific am ...

Oxford University Press2002

Transduction of CD34+ cells with lentiviral vectors enables the production of large quantities of transgene-expressing immature and mature dendritic cells

Didier Trono

BACKGROUND: Genetically engineered dendritic cells (DC) presenting specific antigens to T cells may be of great interest for immunotherapy. For this reason, the production of transgene-expressing DC derived from CD34 + cells transduced either shortly after ...

2001

GFP-expressing mammalian cells for fast, sensitive, noninvasive cell growth assessment in a kinetic mode

Florian Maria Wurm, Martin Jordan, Lisa Hunt

This study correlates the fluorescent signal from stable recombinant CHO cell lines expressing the green fluorescent protein (GFP) at high levels with biomass or cell number, extending the use of fluorescent proteins to applications and assays where cell g ...

1999

Assessment of strain field in endothelial cells subjected to uniaxial deformation of their substrate

Jean-Jacques Meister

A stretch chamber has been developed in order to visualize the deformation of cells subjected to controlled uniaxial stretch of their substrate. A rectangular, custom-made, transparent silicone channel is used as a deformable substrate. Bovine aortic endot ...

Springer Verlag1998

Polymer bound chromogenic crown ethers for optical ion-detection devices

Harm-Anton Klok

The concept of photoinduced electron transfer has been explored for the development of polymeric transducers for optical ion-detection devices. For this purpose, a series of N-(9-anthrylmethyl)monoaza-18-crown-6 substituted poly(methacrylate)s were prepare ...

1996

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