Cellular Uptake and Intracellular Trafficking of Poly(N-(2-Hydroxypropyl) Methacrylamide)
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This thesis describes some of three years'work carried at the Centre Suisse d'Electronique et de Microtechnique (Neuchâtel, Switzerland) under the supervision of Dr. Martha Liley and in collaboration with Prof. Horst Vogel of the Ecole Polytechnique Fédéra ...
A reporter assay was developed to detect and quantify nonsense codon suppression by chem. aminoacylated tRNAs in mammalian cells. It is based on the cellular expression of the enhanced green fluorescent protein (EGFP) as a reporter for the site-specific am ...
BACKGROUND: Genetically engineered dendritic cells (DC) presenting specific antigens to T cells may be of great interest for immunotherapy. For this reason, the production of transgene-expressing DC derived from CD34 + cells transduced either shortly after ...
We present an extremely versatile method for the lateral organization of nano-scale objects (NOs) based on the phenomenon of polymer demixing. NOs are suspended in a soln. of two immiscible polymers, which is used to form a thin polymer film by spin coatin ...
The concept of photoinduced electron transfer has been explored for the development of polymeric transducers for optical ion-detection devices. For this purpose, a series of N-(9-anthrylmethyl)monoaza-18-crown-6 substituted poly(methacrylate)s were prepare ...
This paper describes the development of an analytical technique for arsenic analyses that is based on genetically-modified bioreporter bacteria bearing a gene encoding for the production of a green fluorescent protein (gfp). Upon exposure to arsenic (in th ...
Fluorescent proteins expressed in mammalian cells can be quantified quickly and noninvasively with a standard fluorescence plate reader. We have previously exploited this quality in cell growth assessment (Hunt et al., 1999b). In this work, different CHO c ...
Energy transfer between nanoscale particles is demonstrated for a Gold/rare-earth oxide nanocomposite. The gold nanoparticle acts as an antenna, absorbing light that can then be transferred to the bound rare-earth oxide (see Figure), enhancing the oxide lu ...
This study correlates the fluorescent signal from stable recombinant CHO cell lines expressing the green fluorescent protein (GFP) at high levels with biomass or cell number, extending the use of fluorescent proteins to applications and assays where cell g ...
A stretch chamber has been developed in order to visualize the deformation of cells subjected to controlled uniaxial stretch of their substrate. A rectangular, custom-made, transparent silicone channel is used as a deformable substrate. Bovine aortic endot ...
