Improving 3D MA-TIRF Reconstruction with Deconvolution and Background Estimation
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The SNAP-tag labeling technology provides a simple, robust, and versatile approach to the imaging of fusion proteins for a wide range of experimental applications. Owing to the specific and covalent nature of the labeling reaction, SNAP-tag is well suited ...
G protein-coupled receptors (GPCRs) are ubiquitous mediators of signal transduction across cell membranes and constitute a very important class of therapeutic targets. The main mechanisms involving GPCR activation and signal transduction to the cell interi ...
Localization microscopy such as STORM/PALM achieves the super-resolution by sparsely activating photo-switchable probes. However, to make the activation sparse enough to obtain reconstruction images using conventional algorithms, only small set of probes n ...
Magnetic resonance imaging (MRI) scanners produce raw measurements that are unfit to direct interpretation, unless an algorithmic step, called reconstruction, is introduced. Up to the last decade, this reconstruction was performed by algorithms of moderate ...
This paper presents a method to reconstruct 3D surfaces of silicon wafers from 2D images of printed circuits taken with a scanning electron microscope. Our reconstruction method combines the physical model of the optical acquisition system with prior knowl ...
Membrane protein dynamics is of great importance for living organisms. The precise localization of proteins composing a synapse on the membrane facing a nerve terminus is essential for proper functioning of the nervous system. In muscle fibers, the nicotin ...
Triplet, photo-oxidized and other photoinduced, long-lived states of fluorophores are sensitive to the local environment and thus attractive for microenvironmental imaging purposes. In this work, we introduce an approach where these states are monitored in ...
In this work, we exploit the fact that wavelets can represent magnetic resonance images well, with relatively few coefficients. We use this property to improve magnetic resonance imaging (MRI) reconstructions from undersampled data with arbitrary k-space t ...
Institute of Electrical and Electronics Engineers2011
We present a method for the specific labelling of fusion proteins with synthetic fluorophores in Zebrafish. The method uses the SNAP-tag technology and O-6-benzylguanine derivatives of various synthetic fluorophores. We demonstrate how the method can be us ...
We present a new method for lysis of single cells in continuous flow, where cells are sequentially trapped, lysed and released in an automatic process. Using optimized frequencies, dielectrophoretic trapping allows exposing cells in a reproducible way to h ...