Publications related to Chemistry of Lipid Membranes from Models to Living Systems: A Perspective of Hydration, Surface Potential, Curvature, Confinement and Heterogeneity

Lipid-transfer proteins in biosynthetic pathways

Compartmentalization is a defining feature of eukaryotic cells that allows the spatial segregation of different functions, such as protein and lipid synthesis, and ensures their fidelity and efficiency. This imposes the need for an intense flux of metaboli ...

2008

Making giant unilamellar vesicles via hydration of a lipid film

Suliana Manley

This unit describes protocols for making giant unilamellar vesicles (GUVs) based on rehydration of dried lipid films. These model membranes are useful for determining the impact of membrane and membrane-binding components on lipid bilayer stiffness and pha ...

2008

Evf, a virulence factor produced by the Drosophila pathogen Erwinia carotovora, is an S-palmitoylated protein with a new fold that binds to lipid vesicles

Bruno Lemaitre

Erwinia carotovora are phytopathogenic Gram-negative bacteria of agronomic interest as these bacteria are responsible for fruit soft rot and use insects as dissemination vectors. The Erwinia carotovora carotovora strain 15 (Ecc15) is capable of persisting ...

2008

Imaging of G protein-coupled receptors in solid- supported planar membranes at the single molecule level

Theo Lasser, Marcel Leutenegger

Odorant receptors are an excellent example of natural superiority in specifically binding specific, small and hydrophobic molecules. They are of particular interest in the development of a sensor platform for G protein- coupled receptors (GPCRs). Odorant r ...

SPIE2008

Computer simulation of uranyl uptake by the rough lipopolysaccharide membrane of pseudomonas aeruginosa

Matteo Guglielmi

Heavy metal environmental contaminants cannot be destroyed but require containment, preferably in concentrated form, in a solid or immobile form for recycling or final disposal. Microorganisms are able to take up and deposit high levels of contaminant meta ...

2008

Spontaneous $ Na ^{ + } $ Transients in Individual Mitochondria of Intact Astrocytes

Michaël Unser, Dimitri Nestor Alice Van De Ville, Jean-Yves Chatton

Mitochondria in intact cells maintain low

Na ^{ + }

levels despite the large electrochemical gradient favoring cation influx into the matrix. In addition, they display individual spontaneous transient depolarizations. We report here that individual mit ...

Wiley-Liss2008

Regulation of the V-ATPase along the endocytic pathway occurs through reversible subunit association and membrane localization

Françoise Gisou van der Goot Grunberg, Céline Lafourcade

The lumen of endosomal organelles becomes increasingly acidic when going from the cell surface to lysosomes. Luminal pH thereby regulates important processes such as the release of internalized ligands from their receptor or the activation of lysosomal enz ...

Public Library of Science2008

Chemical Mapping of Ceramide Distribution in Sphingomyelin-Rich Domains in Monolayers

Dusan Vobornik

The incorporation of ceramide in phase-separated monolayers of ternary lipid mixtures has been studied by a combination of atomic force microscopy (AFM), fluorescence, and time-of-flight secondary ion mass spectrometry (ToF-SIMS). Replacement of a fraction ...

American Chemical Society2008

Numerical simulation and optimization of multi-step batch membrane processes

A simple numerical technique is presented for batch membrane filtration design. The underlying model accounts for variable solute rejection coefficients, and it has a modular structure which permits to easily describe the batch process involving different ...

2008

An ultra-thin PDMS membrane as a bio/micro-nano interface: fabrication and characterization

Melody Swartz

We report a method for making ultra-thin PDMS membrane devices. Freely suspended membranes as thin as 70 nm have been fabricated. Bulging tests were performed with a custom built fluidic cell to characterize large circular membranes. The fluidic cell allow ...

Springer Verlag2007

Chemistry of Lipid Membranes from Models to Living Systems: A Perspective of Hydration, Surface Potential, Curvature, Confinement and Heterogeneity

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Lipid-transfer proteins in biosynthetic pathways

Making giant unilamellar vesicles via hydration of a lipid film

Evf, a virulence factor produced by the Drosophila pathogen Erwinia carotovora, is an S-palmitoylated protein with a new fold that binds to lipid vesicles

Imaging of G protein-coupled receptors in solid- supported planar membranes at the single molecule level

Computer simulation of uranyl uptake by the rough lipopolysaccharide membrane of pseudomonas aeruginosa

Spontaneous $ Na ^{ + } $ Transients in Individual Mitochondria of Intact Astrocytes

Regulation of the V-ATPase along the endocytic pathway occurs through reversible subunit association and membrane localization

Chemical Mapping of Ceramide Distribution in Sphingomyelin-Rich Domains in Monolayers

Numerical simulation and optimization of multi-step batch membrane processes

An ultra-thin PDMS membrane as a bio/micro-nano interface: fabrication and characterization

Making giant unilamellar vesicles via hydration of a lipid film

Imaging of G protein-coupled receptors in solid- supported planar membranes at the single molecule level

Regulation of the V-ATPase along the endocytic pathway occurs through reversible subunit association and membrane localization

Spontaneous $ Na ^{ + } $ Transients in Individual Mitochondria of Intact Astrocytes

Lipid-transfer proteins in biosynthetic pathways

Numerical simulation and optimization of multi-step batch membrane processes

An ultra-thin PDMS membrane as a bio/micro-nano interface: fabrication and characterization

Evf, a virulence factor produced by the Drosophila pathogen Erwinia carotovora, is an S-palmitoylated protein with a new fold that binds to lipid vesicles

Chemical Mapping of Ceramide Distribution in Sphingomyelin-Rich Domains in Monolayers

Computer simulation of uranyl uptake by the rough lipopolysaccharide membrane of pseudomonas aeruginosa