Publications related to Photoactivatable Silicon Rhodamines for Super-Resolution Microscopy

Transient State Monitoring by Total Internal Reflection Fluorescence Microscopy

Triplet, photo-oxidized and other photoinduced, long-lived states of fluorophores are sensitive to the local environment and thus attractive for microenvironmental imaging purposes. In this work, we introduce an approach where these states are monitored in ...

2010

A Versatile Route to Red-Emitting Carbopyronine Dyes for Optical Microscopy and Nanoscopy

Marcel Leutenegger

Biological microscopy favors photostable fluorescent markers with large fluorescence quantum yields, low dark triplet state population, good biocompatibility and absorption and emission maxima in the near-infrared, where cellular autofluorescence is minimi ...

2010

Characterization of the cytotoxicity and imaging properties of secondharmonic nanoparticles

Demetri Psaltis, Ye Pu, Rachel Grange, Chia-Lung Hsieh

We develop second-harmonic nanoparticles as the contrast agents for cell imaging. Second-harmonic nanoparticles show promise as cell imaging probes due to their non-bleaching, non-blinking, and coherent signal. Nanoparticles of noncentrosymmetric crystal s ...

Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2010

Three-dimensional nano-localization of single fluorescent emitters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler, Noelia Laura Bocchio

We present a combination of self-interference microscopy with lateral super-resolution microscopy and introduce a novel approach for localizing a single nano-emitter to within a few nanometers in all three dimensions over a large axial range. We demonstrat ...

Optical Society of America2010

Super-resolved position and orientation estimation of fluorescent dipoles using 3D steerable filters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolution by sequentially imaging sparse subsets of fluorophores, which are localized by means of Gaussian-based localization. Applied to fixed fluorophores, with dipole ...

2010

Super-resolved position and orientation estimation of fluorescent dipoles using 3-D steerable filters

Theo Lasser, Iwan Märki, François Aguet, Stefan Geissbühler

The diffraction patterns of fixed fluorophores are characteristic of the orientation of the molecules' underlying dipole. Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolution by sequentially imaging sparse subsets ...

Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa2010

Comparison of Deconvolution Software: A User Point of View—Part 2

Daniel Sage, Alessandra Griffa

Deconvolution is an image processing technique that restores the effective object representation [3] [4], allowing to improve images analysis steps such as segmentation [1] or colocalization study [2]. We performed several deconvolution tests on different ...

EMS2010

Digital holographic microscopy real-time monitoring of cytoarchitectural alterations during simulated microgravity

Christian Depeursinge, Christophe Pache, Jonas Kühn, Muhammed Fatih Toy, Jérôme Parent, Marcel Egli

Previous investigations on mammalian cells have shown that microgravity, either that experienced in space, or simulated on earth, causes severe cellular modifications that compromise tissue determination and function. The aim of this study is to investigat ...

2010

Superresolution Imaging using Single-Molecule Localization

Suliana Manley

Superresolution imaging is a rapidly emerging new field of microscopy that dramatically improves the spatial resolution of light microscopy by over an order of magnitude (similar to 10-20-nm resolution), allowing biological processes to be described at the ...

2010

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Michaël Unser, Matthieu Guerquin-Kern, Ilker Bayram, Raquel Terrés Cristofani

Modern deconvolution algorithms are often specified as minimization problems involving a non-quadratic regularization functional. When the latter is a wavelet-domain

ℓ _{ 1 }

-norm that favors sparse solutions, the problem can be solved by a simple it ...

IEEE2010

Photoactivatable Silicon Rhodamines for Super-Resolution Microscopy

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Transient State Monitoring by Total Internal Reflection Fluorescence Microscopy

A Versatile Route to Red-Emitting Carbopyronine Dyes for Optical Microscopy and Nanoscopy

Characterization of the cytotoxicity and imaging properties of secondharmonic nanoparticles

Three-dimensional nano-localization of single fluorescent emitters

Super-resolved position and orientation estimation of fluorescent dipoles using 3D steerable filters

Super-resolved position and orientation estimation of fluorescent dipoles using 3-D steerable filters

Comparison of Deconvolution Software: A User Point of View—Part 2

Digital holographic microscopy real-time monitoring of cytoarchitectural alterations during simulated microgravity

Superresolution Imaging using Single-Molecule Localization

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Transient State Monitoring by Total Internal Reflection Fluorescence Microscopy

Three-dimensional nano-localization of single fluorescent emitters

Super-resolved position and orientation estimation of fluorescent dipoles using 3-D steerable filters

Super-resolved position and orientation estimation of fluorescent dipoles using 3D steerable filters

A Versatile Route to Red-Emitting Carbopyronine Dyes for Optical Microscopy and Nanoscopy

Superresolution Imaging using Single-Molecule Localization

Characterization of the cytotoxicity and imaging properties of secondharmonic nanoparticles

Accelerated Wavelet-Regularized Deconvolution for 3-D Fluorescence Microcopy

Digital holographic microscopy real-time monitoring of cytoarchitectural alterations during simulated microgravity

Comparison of Deconvolution Software: A User Point of View—Part 2