Publication
Electronic readout of DNA amplification in nanoliter chambers. Strategies towards highly parallel semiconductor-based nucleic acid amplification testing.
Related publications (132)
Development of next-generation microfluidic systems for enhanced, faster, and cost-effective immunoassays for tissue diagnostics - Ac electrothermal flow & Acoustofluidics
The application of microfluidics in the field of surface-based assays and more specifically, the spatial molecular profiling of tumor tissues has gained a lot of interest, especially with the increased interest in personalized medicine and targeted therapy ...
EPFL2023Efficient AC electrothermal flow (ACET) on-chip for enhanced immunoassays
Martinus Gijs, Diego Gabriel Dupouy, Muaz Salama Abdelmonem Draz
Biochemical reaction rates in microfluidic systems are known to be limited by the diffusional transport of reagents, leading often to lowered sensitivity and/or longer detection times in immunoassays. Several methods, including electrically powering electr ...
ROYAL SOC CHEMISTRY2023Spatially multiplexed single-molecule translocations through a nanopore at controlled speeds
Aleksandra Radenovic, Georg Fantner, Vytautas Navikas, Barney Frederick Drake, Helena Miljkovic, Simon Finn Mayer, Sanjin Marion
In current nanopore-based label-free single-molecule sensing technologies, stochastic processes influence the selection of translocating molecule, translocation rate and translocation velocity. As a result, single-molecule translocations are challenging to ...
2023iSort enables automated complex microfluidic droplet sorting in an effort to democratize technology
Christoph Merten, Jatin Panwar
Fluorescence-activated droplet sorting (FADS) is a widely used microfluidic technique for high-throughput screening. However, it requires highly trained specialists to determine optimal sorting parameters, and this results in a large combinatorial space th ...
CELL PRESS2023C2CAplus: A One-Pot Isothermal Circle-to-Circle DNA Amplification System
Sebastian Maerkl, Laura Sophie Grasemann
Rolling circle amplification (RCA) is a widely used DNA amplification method that uses circular template DNA as input and produces multimeric, linear single- or double-stranded DNA. Circle-to-circle amplification (C2CA) has further expanded this method by ...
Washington2023Polydimethylsiloxane microstructure-induced acoustic streaming for enhanced ultrasonic DNA fragmentation on a microfluidic chip
Martinus Gijs, Thomas Lehnert, Lin Sun
Next-generation sequencing (NGS) is an essential technology for DNA identification in genomic research. DNA fragmentation is a critical step for NGS and doing this on-chip is of great interest for future integrated genomic solutions. Here we demonstrate fa ...
ROYAL SOC CHEMISTRY2022Bubble-enhanced ultrasonic microfluidic chip for rapid DNA fragmentation
Martinus Gijs, Thomas Lehnert, Lin Sun
DNA fragmentation is an essential process in developing genetic sequencing strategies, genetic research, as well as for the diagnosis of diseases with a genetic signature like cancer. Efficient on-chip DNA fragmentation protocols would be beneficial to pro ...
ROYAL SOC CHEMISTRY2022Quantification of successful encapsulation into microfluidic compartments
The invention provides a method for quantifying the number of target entities that were successfully encapsulated into a microfluidic compartment such as a microfluidic droplet. The invention is predicated upon a continuous quantifiable detectable signal c ...
2022Enrichment of gut microbiome strains for cultivation-free genome sequencing using droplet microfluidics
We report a droplet microfluidic method to target and sort individual cells directly from complex microbiome samples and to prepare these cells for bulk whole-genome sequencing without cultivation. We characterize this approach by recovering bacteria spike ...
2022Microfluidic systems for in-flow bioanalytes collection
Microfluidic is an ensemble of technologies and methods that permits the handling and analysis of minute volumes of samples in short timescales. Since its introduction it revealed a remarkable potential to unveil novel biological mechanisms. The small volu ...
EPFL2022