Electronic readout of DNA amplification in nanoliter chambers. Strategies towards highly parallel semiconductor-based nucleic acid amplification testing.
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We propose an original concept for a sandwich immunoassay that is completely performed on-chip using streptavidin-coated beads as substrate. The latter are electrostatically self-assembled on aminosilane micropatterns at the bottom of a microfluidic channe ...
Biology has always been a heavily technology limited field. Burgeoning fields such as systems biology require the development and implementation of new technologies, enabling high-throughput and high-fidelity measurements of large systems. Microfluidics pr ...
We propose an original concept for a sandwich immunoassay that is completely performed on-chip using streptavidin-coated beads as substrate. The latter are electrostatically self-assembled on aminosilane micropatterns at the bottom of a microfluidic channe ...
We present and demonstrate a novel technique for the purification of nucleic acids from biological samples using isotachophoresis (ITP). We demonstrate a simple and rapid method to achieve ITP-based extraction, preconcentration, and purification of DNA fro ...
Magnetic microparticles and their application in bioanalytical microfluidic systems have been steadily gaining interest in recent years. This progress is fueled by the comparatively large and long range magnetic forces that can be obtained independently of ...
We report on an innovative approach to on-chip detection of single fluorescently-labeled magnetic micro-particles by means of Single Photon Avalanche Diode (SPAD) arrays. Our system enables particle separation through magnetic actuation and highly sensitiv ...
Polymer-protected sub-2-nm nanogaps that are fabricated via a novel electrical stressing approach exhibit substantial ionic-current reduction in near-physiological conditions. These devices enable direct DNA detection in aqueous solution by utilizing assem ...
Methods for amplification and sequencing of at least one nucleic acid comprising the following steps: (1) forming at least one nucleic acid template comprising the nucleic acid(s) to be amplified or sequenced, wherein said nucleic acid(s) to be amplified o ...
High-throughput, cell-based assays require small sample volumes to reduce assay costs and to allow for rapid sample manipulation. However, further miniaturization of conventional microtiter plate technology is problematic due to evaporation and capillary a ...
This thesis reports on the development of technologies and methods for a microfiuidic device for continuous pressure-driven flow-injection analysis. Chip based, pressure-driven liquid chromatographic (LC) systems are often avoided due to the difficulties e ...