Engineering of the human O6-alkylguanine DNA alkyltransferase
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Deutsche Akademie der Naturforscher Leopoldina2001
The design and synthesis of chem. probes to study and to elucidate complex biol. problems is becoming an increasingly important field in chem. We are interested in the repair of O6-alkylated guanines in DNA, a DNA lesion that results from alkylation by S-a ...
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A general method for the specific and covalent labeling of fusion proteins in vivo is described. The approach is based on the unusual mechanism of the human DNA repair protein O6-alkylguanine-DNA alkyl-transferase, which irreversibly transfers an alkyl gro ...
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(Ecole Polytechnique Federale De Lausanne, Switz.).2002
