Engineering of the human O6-alkylguanine DNA alkyltransferase
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Characterizing the movement, interactions, and chem. microenvironment of a protein inside the living cell is crucial to a detailed understanding of its function. Most strategies aimed at realizing this objective are based on genetically fusing the protein ...
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A general method for the specific and covalent labeling of fusion proteins in vivo is described. The approach is based on the unusual mechanism of the human DNA repair protein O6-alkylguanine-DNA alkyl-transferase, which irreversibly transfers an alkyl gro ...
We combined biophys., biochem., and pharmacol. approaches to investigate the ability of the a1a- and a1b-adrenergic receptor (AR) subtypes to form homo- and hetero-oligomers. Receptors tagged with different epitopes (hemagglutinin and Myc) or fluorescent p ...
Studying proteins in living cells generally requires the construction of a fusion protein between the protein of interest and an appropriate tag. Currently used fusion protein-based assays rely on the genetically encoded properties of the tag, which signif ...
A method using O6-alkylguanine-DNA alkyltransferases (AGT) is disclosed for transferring a label from a substrate to a fusion protein comprising the AGT. This allows the detection and/or manipulating of the fusion protein, both in vitro and in vivo, by att ...
(Ecole Polytechnique Federale De Lausanne, Switz.).2002
An extensive random mutagenesis study on the function of nine residues in the subunit binding site of FimC was conducted to evaluate the mol. mechanisms of subunit binding by FimC and the contribution of chaperone surface residues. Five plasmid libraries o ...
Deutsche Akademie der Naturforscher Leopoldina2001
The design and synthesis of chem. probes to study and to elucidate complex biol. problems is becoming an increasingly important field in chem. We are interested in the repair of O6-alkylated guanines in DNA, a DNA lesion that results from alkylation by S-a ...
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A review with 100 refs. The enormous potential of combinatorial approaches for studying problems in biol. and chem. has been clearly demonstrated over the last several years. In particular, phage display has become one of the major techniques for the use o ...