Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba

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Biophysical applications of correlative scanning probe and super-resolution microscopy

Imaging live cells in their native environment is crucial for the understanding of complex biological phenomena. Modern optical microscopy methods such as fluorescence super-resolution microscopy are increasingly combined with complementary, label-free tec ...

EPFL2021

Intraocular Lens Refractive Index and Its Impact on External Surface Reflections

Georges Wagnières

PURPOSE: To determine and compare the origin of the external surface reflections produced by commonly used intraocular lenses (IOLs). ...

SLACK INC2021

Structured light generation by refractive-diffractive micro optical array illuminators

Maryam Yousefi

Structured light generation having broad applications in different optical fields, is the topic of this thesis. Our structured light generation strategy is based on applying periodic microoptical elements at the refraction-diffraction limit, under a focuse ...

EPFL2021

Optical diffraction tomography from single-molecule localization microscopy

Michaël Unser, Thanh-An Michel Pham, Emmanuel Emilien Louis Soubies, Ferréol Arnaud Marie Soulez

Single-molecule localization microscopy (SMLM) is a powerful method for the imaging of cellular structures. This modality delivers nanoscale resolution by sequentially activating a subset of fluorescent molecules and by extracting their super-resolved posi ...

2021

Diffraction Tomography from Single-Molecule Localization Microscopy: Numerical Feasibility

Michaël Unser, Thanh-An Michel Pham, Emmanuel Emilien Louis Soubies, Ferréol Arnaud Marie Soulez

Single-molecule localization microscopy (SMLM) is a fluorescence microscopy technique that achieves super-resolution imaging by sequentially activating and localizing random sparse subsets of fluorophores. Each activated fluorophore emits light that then s ...

IEEE2021

ON THE DESIGN AND OPTIMIZATION OF WIDE ANGLE DIFFRACTIVE OPTICAL ELEMENTS

Dong Cheon Kim

Diffractive optical elements (DOEs) which consist of microstructure surface relief permit the generation of the spatial distribution of light beams by using a single element. Due to their compact size, design flexibility, and mass productivity, they are us ...

EPFL2021

Learning Lipschitz-Controlled Activation Functions in Neural Networks for Plug-and-Play Image Reconstruction Methods

Michaël Unser, Dimitris Perdios, Pakshal Narendra Bohra, Alexis Marie Frederic Goujon, Sébastien Alexandre Emery

Ill-posed linear inverse problems are frequently encountered in image reconstruction tasks. Image reconstruction methods that combine the Plug-and-Play (PnP) priors framework with convolutional neural network (CNN) based denoisers have shown impressive per ...

2021

In-operando dynamic visualization of flow through porous preforms based on X-ray phase contrast imaging

Véronique Michaud, Baris Çaglar, Helena Luisa Teixido Pedarros

Direct visualization is often sought to elucidate flow patterns and validate models to predict the filling kinetics during processes whereby a liquid resin infiltrates a textile porous preform. Here, X-ray phase contrast interferometry is evaluated to imag ...

2021

Development of novel experimental and computational methods for three-dimensional coherent and super-resolution microscopy

Adrien Charles-François Raymond Descloux

Optical microscopy is one widely used tool to study cell functions and the interaction of molecules at a sub-cellular level. Optical microscopy techniques can be broadly divided into two categories: partially coherent and incoherent. Coherent microscopy te ...

EPFL2020

Three-dimensional tomography of red blood cells using deep learning

Demetri Psaltis, Joowon Lim, Ahmed Ayoub

We accurately reconstruct three-dimensional (3-D) refractive index (RI) distributions from highly ill-posed two-dimensional (2-D) measurements using a deep neural network (DNN). Strong distortions are introduced on reconstructions obtained by the Wolf tran ...

2020

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Intraocular Lens Refractive Index and Its Impact on External Surface Reflections

Georges Wagnières

PURPOSE: To determine and compare the origin of the external surface reflections produced by commonly used intraocular lenses (IOLs). ...

SLACK INC2021

Development of novel experimental and computational methods for three-dimensional coherent and super-resolution microscopy

Adrien Charles-François Raymond Descloux

EPFL2020