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Tryptic digestion of proteins in trypsin loaded porous silica has been shown to be highly efficient. Enzymatic silica-reactors were prepared by immobilizing trypsin into macroporous ordered siliceous foam (MOSF) and into mesoporous SBA-15 silica which has ...
The use of zeolites for catalytic reactions is a field in continuous development. Since it was demonstrated that metal-zeolites are efficient catalysts for NOx abatement, decomposition of nitrous oxide (N2O) over Fe-containing zeolites has attracted great ...
Surfactant liposome interactions have been previously studied through different methods and techniques. We present here a classical physical chemistry study on liposome solutions added to destabilizing agents at concentrations well above the solubilization ...
The objective of this thesis is the development of a compact reactor for continuous three-phase reactions in single-pass configurations. The design is based on a bubble column reactor staged with layers of catalytic material. To make the reactor suitable f ...
Proteolysis with proteases preloaded within nanopores of porous material is a very fast process, where proteins can be digested in minutes compared to the conventional bulk enzyme reactions taking place over hours. To model this surprising phenomenon, a mo ...
The time-dependent behavior of sensor systems based on enzyme membranes is treated theoretically. The comparative treatment covers traditional potentiometric and amperometric enzyme electrodes as well as enzyme reactors. Theoretical solutions are derived b ...
A novel one-step microfabrication process of SU-8 films for the development of inexpensive light-weight and small size optical glucose micro-biosensors is proposed. Experimental work was carried out in order to investigate whether SU-8 photoresists, which ...
Nanoliter plates are versatile tools for the screening of split-and-mix libraries both on-bead and off-bead. The method was evaluated for the identification of hydrolytically active compounds and enzyme inhibitors. ...
During the last 25 years, display techniques such as phage display have become very powerful tools for protein engineering, especially for the selection of monoclonal antibodies. However, while this method is extremely efficient for affinity-based selectio ...
A novel nanopore based digestion strategy has been developed by directly adding a macroporous material as catalyst to the conventional in-solution reaction system. Without increasing the enzyme or protein concentrations, this simple digestion approach exhi ...