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Bicyclic peptides are small, constrained peptides that can bind with high affinity and selectivity to protein targets. Their small size provides a number of advantages over larger protein-based ligands, including access to chemical synthesis, better tissue ...
DNA biosensing requires high oligonudeotide binding capacity interface chemistries that can be tuned to maximize probe presentation as well as hybridization efficiency. This contribution investigates the feasibility of aldehyde-functionalized poly(2-hydrox ...
While antibodies currently play a dominant role as affinity reagents in biological research and for diagnostics, a broad range of recombinant proteins are emerging as promising alternative affinity reagents in detection assays and quantification. DNA-media ...
The precise tuning of gene expression levels is essential for optimal performance of transcriptional regulatory networks. Many regulatory components control promoter output, but transcription factor (TF) binding sites are amongst the most prominent. Beside ...
An integrated chip for DNA hybridization detection was realized in a standard CMOS process: it hosts 80 biosensors subdivided in 2 channels, as well as D/A and A/D converters for electrical stimulation and readout. A microfluidic system, bonded on the surf ...
Methods to manipulate and visualize isolated DNA and oligonucleotide strands are important for investigation of their biophysics as well as their interactions with proteins. Herein, we report such a method by combining a block copolymer surface functionali ...
A unique and practical synthetic sequence for rapid access to polyketides and to further the spiroacetals derived from them, which utilizes a bidirectional Hosomi-Sakurai allylation approach around key allylsilanes in the synthesis of the AB and CD ring sy ...
We describe a new method for the solvent-free synthesis of borohydrides at room temperature and demonstrate its feasibility by the synthesis of three of the most discussed borohydrides at present: LiBH4, Mg(BH 4)2 and Ca(BH4)2. This new gas-solid mechanoch ...
Detection of non-self RNA by TLRs within endosomes and by retinoic acid-inducible gene I (RIG-I)-like helicases in the cytosol is central to mammalian antiviral immunity. In this study, we used pathway-specific agonists and targeted delivery to address RNA ...
The invention relates to a method of preparing template constructs for solid-phase nucleic acid amplification and to use of the templates in methods of solid-phase nucleic acid amplification. The method involves carrying out two ligation reactions: (a) a l ...