Concept

Oil immersion

Publications associées (94)

Digital Holographic Microscopy Applied to Diffraction Tomography of a Cell Refractive Index

Christian Depeursinge, Tristan Colomb, Etienne Cuche, Pierre Marquet, Florian Charrière

We present a approach of 3-dimensional refractive index tomography of biological specimen, based on the high accuracy phase measurement provided by Digital Holographic Microscopy, recorded for a complete rotation of the specimen in the setup. ...

OSA2006

Fast focus field calculations

Theo Lasser, Marcel Leutenegger, Rainer Leitgeb, Ramachandra Rao

We present a fast calculation of the electromagnetic field near the focus of an objective with a high numerical aperture (NA). Instead of direct integration, the vectorial Debye diffraction integral is evaluated with the fast Fourier transform for calculat ...

2006

PhotoMEA: development of optical tools for the study of the functional properties of neuronal networks

Diego Ghezzi

A large number of studies on neuronal physiology and plasticity have provided a detailed picture of the molecular machinery underlying the modulation of neuronal activity. On the contrary, the mechanisms controlling properties of complex neuronal networks ...

2006

Extension of the Williams-Clapper model to stacked nondiffusing colored coatings with different refractive indices

Roger Hersch, Mathieu Hébert

We propose a model for predicting the reflectance and transmittance of multiple stacked nonscattering coloring layers that have different refractive indices. The model relies on the modeling of the reflectance and transmittance of a bounded coloring layer, ...

Opt. Soc. America2006

Solid immersion lens used in a microscope with a lens system is made of a material having a refractive index that is selected so that the numerical aperture of the lens system

Theo Lasser, Ramachandra Rao

The invention relates to a solid immersion lens (SIL) for a microscope, comprising a lens system with a predetermined numerical aperture. The refractive index of the material of the solid immersion lens is selected in such a way that the numerical aperture ...

2005

Total internal reflection fluorescence correlation spectroscopy (TIR-FCS) with low background and high count-rate per molecule

Theo Lasser, Marcel Leutenegger, Kai Hassler, Ramachandra Rao

We designed a fluorescence correlation spectroscopy (FCS) system for measurements on surfaces. The system consists of an objective-type total internal reflection fluorescence (TIRF) microscopy setup, adapted to measure FCS. Here, the fluorescence exciting ...

2005

Measurement and interpretation of the 3D amplitude point spread function of lenses and microscope objectives

The intensity point spread function (IPSF) is most often used to characterize optical imaging systems. For some imaging applications such as digital holographic microscopy the 3D amplitude point spread function (APSF) more completely describes an optical s ...

EPFL2005

Polarization control and sensing with two-dimensional coupled photonic crystal microcavity arrays

Hatice Altug

We have experimentally studied polarization properties of the two-dimensional coupled photonic crystal microcavity arrays and observed a strong polarization dependence of the transmission and reflection of light from the structures-effects that can be empl ...

2005

Fabrication of optical planar waveguides in KY(WO4)2 by He-ion implantation

René Salathé, Camelia Borca

In this paper, planar waveguides produced by He-ion implantation have been demonstrated in undoped and Yb-doped KY(WO4)2 crystals. The effective refractive indices of guided modes in surface planar waveguides were measured by dark m-line spectroscopy and t ...

IEEE2005

High light field confinement for Fluorescence Correlation Spectroscopy using a Solid Immersion Lens

Theo Lasser, Denis Martin, Ramachandra Rao

In this paper we present recent single molecule detection experiment using a solid immersion lens (SIL) for fluorescent correlation spectroscopy measurements. We compared the performance of the SIL in combination with an air objective (40×, numerical apert ...

2004

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