Publications associées à Test immunologique

Controlling the immobilization process of an optically enhanced protein microarray for highly reproducible immunoassay

By virtue of its high throughput multiplex detection capability, superior read-out sensitivity, and tiny analyte consumption, an optically enhanced protein microarray assay has been developed as a promising diagnostic tool for various applications, ranging ...

ROYAL SOC CHEMISTRY2021

Rapid Multianalyte Microfluidic Homogeneous Immunoassay on Electrokinetically Driven Beads

Carlotta Guiducci, Pierre-Emmanuel Marie Thiriet, Gloria Porro, Danashi Imani Medagoda

The simplicity of homogeneous immunoassays makes them suitable for diagnostics of acute conditions. Indeed, the absence of washing steps reduces the binding reaction duration and favors a rapid and compact device, a critical asset for patients experiencing ...

2020

Memristive Biosensors based on Full-size Antibodies and Antibody Fragments

Giovanni De Micheli, Ioulia Tzouvadaki, Sandro Carrara, Sébastien Naus

For the first time memristive biosensors developed by the incorporation of scAbs (single-chain antibody fragments) enabling label-free detection of free Prostate Specific Antigen (fPSA) for early-stage detection of prostate cancer (PC) are reported. In add ...

2019

Microdevice-integrated immunoassays for Point-of-Care Therapeutic Drug Monitoring

Elena-Diana Bojescu

A significant number of drugs exhibit narrow therapeutic windows and high inter-individual pharmacokinetic variability. In such cases, Therapeutic Drug Monitoring (TDM) is compulsory in order to optimize the efficacy of the drug while avoiding adverse effe ...

EPFL2018

Multiplexed nanoplasmonic biosensor for one-step simultaneous detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine

Hatice Altug, Maria Soler Aznar, Andrea Cavallini, Alexander Belushkin

Development of rapid and multiplexed diagnostic tools is a top priority to address the current epidemic problem of sexually transmitted diseases. Here we introduce a novel nanoplasmonic biosensor for simultaneous detection of the two most common bacterial ...

2017

Alkaline phosphatase-fused repebody as a new format of immuno-reagent for an immunoassay

Oliver Hantschel

Enzyme-linked immunoassays based on an antibody-antigen interaction are widely used in biological and medical sciences. However, the conjugation of an enzyme to antibodies needs an additional chemical process, usually resulting in randomly cross-linked mol ...

Elsevier2016

Fabrication of a high aspect ratio (HAR) micropillar filter for a magnetic bead-based immunoassay

Martinus Gijs, Christine Ruffert

A microfluidic chip has been realized for investigating immune cell (U937) activation with lipopolysaccharide (LPS) and subsequent pro-inflammatory cytokine (Interleukin-6, IL-6) detection (Ruffert et al. Proc. EMBL Conference Microfluidics 2012a, p 184; P ...

Springer2014

Total serum IgE quantification by microfluidic ELISA using magnetic beads

Hubert Girault, Anne-Laure Gassner

The present work reports on the quantification of total IgE in human serum using a microanalytical device whose fluidics is driven by gravity and capillary forces only. Thanks to the eight parallel microchannels in each microchip, calibration and sample an ...

Springer Verlag2012

Capillary Microfluidic Chips for Point-of-Care Testing : from Research Tools to Decentralized Medical Diagnostics

Luc Gervais

Research on microfluidic devices for biological analysis has progressed sufficiently to be developed into point-of-care diagnostics products. The goal of this thesis is to improve multiple aspects of capillary-driven microfluidic devices. In particular, th ...

EPFL2011

MULTI-ANALYTE DETECTION FOR BIOLOGICAL FLUIDS Towards Continous Monitoring of Glucose, Ionized Calcium and pH using a Viscometric Affinity Biosensor

Peter Ryser, Eric Meurville, Christophe Boss, Frédéric Schmitt, Pablo Dosil Rosende

We present a viscometric affinity biosensor that can potentially allow continuous multi-analyte monitoring in biological fluids like blood or plasma. The sensing principle is based on the detection of viscosity changes of a polymeric solution which has a s ...

SciTePress2011