Identité de genre | EPFL Graph Search

alt=Représentation de l'identité de genre sous la forme d'un spectre carré. Les personnes hommes et femmes sont représentées dans deux coins opposés. L'absence d'identité de genre occupe le coin en bas à gauche. Le coin en haut à droite représentent les identités de genre qui ne sont pas sur le spectre homme-femme.|vignette|Représentation de différentes identités de genre sous la forme d'un spectre à deux dimensions L'identité de genre (parfois identité sexuelle ou identité sexuée) d'une personne est la catégorie de genre à laquelle elle estime appartenir. On peut par exemple se concevoir comme une femme, un homme, ou une personne non-binaire. Dans le cas où l'identité de genre correspond au genre assigné à la naissance, on parle de cisidentité ou de personne cisgenre. Dans le cas contraire, on parle de transidentité ou de personne transgenre. Dans les années 1960, de premières études de psychologie décrivent comment l'identité de genre se développe au cours de l'enfance. Ces études p

L'oeuvre de Leandro V. Locsin (1928-1994), architecte à la recherche de l'identité élusive de l'architecture philippine du second après-guerre

Jean-Claude Girard

Filipino architect Leandro Valencia Locsin (Silay1928 - Makati 1994) produced an architectural work that involved 250 projects, of which at least more than half were completed and mostly in his country of origin. A graduate in architecture of San Tomas de Manila University in 1953, his career began after the Second World War, which left the capital city all but destroyed following airstrikes by the allies that served to liberate the Philippines from the Japanese occupying forces. Following his studies, Locsin is immediately solicited with requests which leads him to open his office and give up the idea of completing his graduate studies in one of the prestigious American universities, which at the time had welcomed great masters who had sought exile from Europe to the United States in the 1930s. For a member of a privileged social class, this atypical career path makes Locsin a singular figure in the architectural landscape as he will immediately focus his interest on questioning the specificity of Filipino architecture. In a 1989 interview, Locsin confirms his career-long interest in researching the boundaries between local culture and international influences. The study of the vernacular tradition in his country will orient his work towards defining certain architectural principles to allow for the emergence of a new contemporary Filipino architecture. The post-war period, involving innovations in technical resources and favorable political and economic conditions for urban development, will serve to render all the more complex a body of work that is difficult to apprehend and qualify. In a 1964 text, Locsin refers to the elusive identity of the Filipino architectural spirit which, according to him, is the result of an assimilation of various external influences, given the geographic positioning of the Philippines and its colonial past. His work never ceased to reflect this quest for identity in built representations. While Leandro V. Locsin has been called "the poet of space" for the quality of his projects characterized by a great attention given to the sense of space, abstraction, symmetry, proportion, and materiality linked to the use of concrete and traditional materials his work has never been the subject of a complete study. Few scientific contributions exist and remain partial, in that they focus on certain specificities of his production. Further, there are no studies that offer a complete review of his office archives, a necessary step towards uncovering and highlighting the unique characteristics of a multiform production. The objective of this dissertation is to provide a complete overview of the work of Leandro V. Locsin, notably through a survey of his projects, and an analysis of the condition of their production in a local and international context, in order to render explicit the specific qualities that make this work a unique example of architecture of the period and from the Philippines.

EPFL2018

MATERIAL POINT METHOD A numerical evaluation for elastic problems

Mathilde Metral

Frozen, the movie, released in 2013, made a big impression on many people: some children were no doubt amazed by the talking snowman, others by the film’s music, the designers were probably more interesed in the reproduction quality of the snow modelled, by the Material Point Method (MPM). Many paperspraise the effectiveness of this method for simulating complex phenomena. However, very few studies have been carried out to verify the method on very simple academic problems; that is precisely what we will try to study throughout this paper. We will see that simple problem modeling is not so easy with MPM. We will try to present as well as possible the different factors influencing the results, before concluding on its advantages and disadvantages

2020

Human U6 promoter drives stronger shRNA activity than its schistosome orthologue in Schistosoma mansoni and human fibrosarcoma cells

Raphaël Duvoisin, Nicola Harris

Blood flukes or schistosomes are the causative agents of human schistosomiasis, one of the major neglected tropical diseases. Draft genome sequences have been reported for schistosomes, but functional genomics tools are needed to investigate the role and essentiality of the newly reported genes. Vector based RNA interference can contribute to functional genomics analysis for schistosomes. Using mRNA encoding reporter firefly luciferase as a model target, we compared the performance of a schistosome and a human promoter from the U6 gene in driving shRNA in human fibrosarcoma cells and in cultured schistosomes. Further, both a retroviral [Murine leukemia virus (MLV)] and plasmid (piggyBac, pXL-Bac II) vector were utilized. The schistosome U6 gene promoter was 270 bp in length, the human U6 gene promoter was 264 bp; they shared 41% identity. Following transduction of both HT1080 fibrosarcoma cells and schistosomules of Schistosoma mansoni with pseudotyped MLV virions, stronger knockdown of luciferase activity was seen with the virions encoding the human U6 promoter driven shRNA than the schistosome U6 promoter. A similar trend was seen after transfection of HT1080 cells and schistosomules with the pXL-Bac-II constructs-stronger knockdown of luciferase activity was seen with constructs encoding the human compared to schistosome U6 promoter. The findings indicate that a human U6 gene promoter drives stronger shRNA activity than its schistosome orthologue, not only in a human cancer cell line but also in larval schistosomes. This RNA polymerase III promoter represents a potentially valuable component for vector based RNA interference studies in schistosomes and related platyhelminth parasites.

2011

L'oeuvre de Leandro V. Locsin (1928-1994), architecte à la recherche de l'identité élusive de l'architecture philippine du second après-guerre

Jean-Claude Girard

EPFL2018