Concept

Hybridation in situ en fluorescence

Publications associées (222)

Aerobic granular sludge reactor technology Microbial diversity of aerobic granules

• Goal The goal of the project was to produce biomolecular fingerprints in order to describe the structure of microbial community which develops during aerobic sludge granulation, initiated in sequencing batch wastewater treatment processes. Four SBR were ...

2006

Development and functionalisation of Sb doped SnO2 thin films for DNA biochip applications

Carlotta Guiducci, Olivier Renault

In view of developing DNA biochips based on electric detection, well controlled DNA functionalisation of conductive substrate electrode is a challenging process. In this study, the whole process of functionalisation onto home made conductive films with con ...

2006

CMOS DNA sensor array with integratedA/D conversion based on label-free capacitance measurement

Sandro Carrara, Carlotta Guiducci

This paper presents a fully electronic label-free DNA chip in 0.5-mu m CMOS technology, with 5-V supply voltage, suitable for low-cost highly integrated applications. The chip features an array of 128 sensor sites with gold electrodes and integrated measur ...

2006

Micro-array device, for DNA recognition, includes micro-sensors to output signals indicating absorption of ultraviolet radiation in micro-location, when traversed by UV beam during hybridization process

Carlotta Guiducci

NOVELTY - The micro-array device has several micro-locations (3) associated with a preset DNA sequence. Several micro-sensors are positioned corresponding to the micro-locations. The micro-sensors output electrical signals indicating the absorption of ultr ...

Univ Alma Mater Studiorum Di Bologna; Ricco B; Guiducci C2006

Improvement of the contrast in cancer detection by autofluorescence bronchoscopy using a narrow spectral violet excitation: A preliminary study

Hubert van den Bergh, Georges Wagnières, Tanja Gabrecht, Blaise Lovisa

Autofluorescence (AF) bronchoscopy is a useful tool for early cancer detection. However the mechanisms involved in this diagnosis procedure are poorly understood. We present a clinical autofluorescence imaging study to assess the depth of the principal con ...

2006

Dual-color Total Internal Reflection Fluorescence Fluctuation Spectroscopy

Theo Lasser, Marcel Leutenegger

We developed a dual-color total internal reflection fluorescence (TIRF) system for single molecule imaging and fluorescence fluctuation spectroscopy (FFS). For a performance analysis, we measured a synthetic binding assay with dual-color global fluorescenc ...

2006

Dual-color Total Internal Reflection Fluorescence Fluctuation Spectroscopy

Theo Lasser, Marcel Leutenegger

2006

Covalent labeling of cell-surface proteins for in-vivo FRET studies

Horst Vogel, Kai Johnsson, Rudolf Hovius, Jean-Manuel Segura, Nathalie George, Pedro Pascoal, Bruno Meyer

Fluorescence resonance energy transfer (FRET) is a powerful technique to reveal interactions between membrane proteins in live cells. Fluorescence labeling for FRET is typically performed by fusion with fluorescent proteins (FP) with the drawbacks of a lim ...

2006

Systemic Distribution and Elimination of Plain and with Cy3.5 Functionalized Poly(vinyl alcohol) Coated Superparamagnetic Maghemite Nanoparticles After Intraarticular Injection in Sheep In Vivo

Heinrich Hofmann, Alke Fink, Benedikt Steitz

PVA coated and fluorescent dye (Cy3.5) functionalized vinyl alcohol/vinyl amine copolymer coated superparamagnetic iron oxide nanoparticles (SPION) were evaluated for systemic distribution and elimination after intraarticular injection in sheep. Observatio ...

2006

Hardware-software design of a smart sensor for fully-electronic DNA hybridization detection

Carlotta Guiducci

This paper describes the design of a smart sensor for label-free detection of DNA hybridization. The sensor is based on a direct electrical transduction principle: it measures impedance variation at the interface between a bio-functionalized electrode and ...

2005

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