Endoscopie | EPFL Graph Search

L'endoscopie ou fibroscopie est une méthode d'exploration visuelle médicale ou industrielle de l'intérieur (endon en grec) d'une cavité inaccessible à l'œil. L'instrument utilisé, appelé endoscope ou fibroscope, est composé d'un tube optique muni d'un système d'éclairage. Lorsqu'il est couplé à une caméra vidéo, il peut retransmettre l'image sur un écran. L'endoscopie consiste en des examens spécifiques par organe. Elle peut être utilisée soit pour un diagnostic, soit pour une intervention (endoscopie chirurgicale). Ainsi, la bronchoscopie est l'exploration des bronches, la coloscopie celle du côlon, la cystoscopie celle de la vessie, la gastroscopie celle de l'œsophage ou de l'estomac, la cœlioscopie celle de l'abdomen, etc. Elles se font par les voies naturelles lorsque cela est possible, autrement par incision. L'endoscopie industrielle, quant à elle, regroupe trois technologies : la borosocopie, la flexoscopie et la vidéoscopie, et est couramment utilisée pour le contrôle inte

Endoscopic low coherence interferometry applied to tri-dimensional contouring of upper airways

Yves Delacrétaz

This thesis presents a novel approach for optical tri-dimensional contouring, requiring only one acquisition to extract a contour depth. It is based on an interferometric setup, using a reduced coherence light source, and is fully adaptable to endoscopic procedure commonly in used in ear-nose-throat (ENT) medicine. It has been demonstrated that the method can be successfully applied to imaging of porcine upper airways. Although this method uses interferometry to obtain depth range measurement, the phase signal is not directly evaluated, rather the location of the coherent superposition of two waves is extracted, and thus provides a robust technique even in harsh environment. The key feature is to extract the coherent area on the image by locating intentionally induced fringes created by off-axis superposition of a smooth reference wave and an object wave coming from a rough or diffusing sample through an optical system with limited aperture. A model for the propagation of the light through the optical system has been developed. It takes into account the broadened emission spectrum of the light source, as well as the statistical speckle effect induced by the illumination of a rough surface with partially coherent light. Simulated results have been carefully compared with measurements, both in the spatial domain and in the Fourier domain (spatial frequencies response), which has permitted to better understand the processes involved in the creation of the interference fringes, and improve the design of the device. Different filters have been investigated in order to extract the depth information from acquired interferograms. By using Gabor filterbanks, which do not need any a priori knowledge of the signal, selectivity of the extracted signal has been enhanced by a factor of 1.3 compared to a simple local Fourier spectrum evaluation, and time of the extraction procedure divided by 6, even when compared to fast iterative calculation in the Fourier spectrum. Moreover, by imposing the frequency and/or orientation of the signal to detect, which can be known by a simple calibration procedure, it has been proved that the selectivity can be further enhanced by a factor of 2.5, keeping the calculation time as short or even shorter. A compact prototype has been designed and built, with a specific multiple fibers arrangement for illumination. The system is separated in two parts: the first one is directly attached to the rigid endoscope, and contains imaging as well as recombining object/reference wave optics, and the detector. It fits into a box with overall dimensions of 115 × 83 × 94 mm. The second part contains the laser source, the scanning arm and the injection optics for the fibered system. It is designed to be left on a small cart. This device can potentially be brought to the bedside, and used during routine endoscopic check-up. Associated with the device, a complete framework has been elaborated in order to simplify the acquisition procedure as well as the signal processing. This results in a fully automated environment taking in charge hardware control, data storage and signal processing, up to the tri-dimensional scene rendering.

EPFL2009

Multimode fiber optical imaging using wavefront control

Nicolino Stasio

Optical microscopy enables us to observe at high resolution and with a large field of view objects otherwise invisible for the naked eye. This is why it is becoming a fundamental tool in biology and in diagnostics, where observation at cellular level can tell about the health status of a tissue. At visible wavelengths, light undergoes scattering when propagating through tissues, limiting the imaging depth to the first cellular layers of a biological sample. Endoscopic approaches allow the bypass of the scattering layers and move the accessible volume centimeters inside tissues, ideally keeping large field of view and high-resolution capabilities. In this thesis we explore the combination of wavefront control and ultrathin multimode optical fibers to develop a new family of endoscopes able to generate images with micrometric resolution being, at the same time, minimally invasive. We demonstrate that a variety of imaging techniques can be implemented in optical fiber-based endoscopy, maximizing the amount of information that can be collected through a few hundreds of micrometers-thick probe. Firstly, we describe digital phase conjugation (DPC), which is the utilized wavefront shaping technique to convert a multimode optical fiber in an ultrathin endoscope. The use of DPC in synergy with multimode optical fibers resulted in ultrathin probes able to perform fluorescence imaging, photoacoustic imaging, and multiphoton imaging. Moreover, we show that using saturation excitation of fluorescent samples we can beat the resolution limit imposed by the numerical aperture of the optical fiber and obtain optical sectioning. In the first part of the thesis we describe which steps have been performed towards the implementation of these techniques. In the second part, we exploit coherent fiber bundles as imaging probes. This kind of fiber is typically utilized in endoscopy, but their resolution is limited by the distance between the fiberâs cores. Here we show two techniques - one based on DPC and a second based on statistical properties of speckle patterns - to obtain images with a better resolution than the one imposed by the core-to-core spacing, both relying on a unique property of multicore fibers: the memory effect. We also accomplished complex pattern transmission through multicore fibers with high resolution. Overall, the presented work provides new insights in imaging through optical fibers based on wavefront shaping techniques, opening up pathways for addressing current issues in this research field.

EPFL2017

Endoscopic low coherence interferometry in upper airways

Daniel Boss, Yves Delacrétaz, Christian Depeursinge, Florian Lang

We introduce Endoscopic Low Coherence Interferometry to obtain topology of upper airways through commonly used rigid endoscopes. Quantitative dimensioning of upper airways pathologies is crucial to provide maximum health recovery chances, for example in order to choose the correct stent to treat endoluminal obstructing pathologies. Our device is fully compatible with procedures used in day-to-day examinations and can potentially be brought to bedside. Besides this, the approach described here can be almost straightforwardly adapted to other endoscopy-related field of interest, such as gastroscopy and arthroscopy. The principle of the method is first exposed, then filtering procedure used to extract the depth information is described. Finally, demonstration of the method ability to operate on biological samples is assessed through measurements on ex-vivo pork bronchi.

SPIE2009