Capillary electrophoresis–mass spectrometry

Résumé

Capillary electrophoresis–mass spectrometry (CE–MS) is an analytical chemistry technique formed by the combination of the liquid separation process of capillary electrophoresis with mass spectrometry. CE–MS combines advantages of both CE and MS to provide high separation efficiency and molecular mass information in a single analysis. It has high resolving power and sensitivity, requires minimal volume (several nanoliters) and can analyze at high speed. Ions are typically formed by electrospray ionization, but they can also be formed by matrix-assisted laser desorption/ionization or other ionization techniques. It has applications in basic research in proteomics and quantitative analysis of biomolecules as well as in clinical medicine. Since its introduction in 1987, new developments and applications have made CE-MS a powerful separation and identification technique. Use of CE–MS has increased for protein and peptides analysis and other biomolecules. However, the development of online

Source officielle

https://en.wikipedia.org/wiki/Capillary_electrophoresis%E2%80%93mass_spectrometry

À propos de ce résultat

Cette page est générée automatiquement et peut contenir des informations qui ne sont pas correctes, complètes, à jour ou pertinentes par rapport à votre recherche. Il en va de même pour toutes les autres pages de ce site. Veillez à vérifier les informations auprès des sources officielles de l'EPFL.

Publications associées (10)

Publications associées

Chargement

Personnes associées (1)

Personnes associées

Chargement

Unités associées

Chargement

Concepts associés (2)

Concepts associés

Chargement

Cours associés (1)

Les étudiants comprennent les bases physico-chimiques des méthodes de séparation chromatographiques et électrophorétiques.

Cours associés

Chargement

Séances de cours associées (1)

Séances de cours associées

Chargement

Etude et illustration de méthodes itératives d'optimisation non linéaire

Thierno Diallo

Il s'agira d'étudier un certain nombre de méthodes classiques basées notamment sur les gradients des fonctions à optimiser; ces techniques seront analysées du point de vue de leur convergence et le candidat aura pour tâche de les mettre en oeuvre en les programmant, de les comparer et d'élaborer un outil graphique permettant d'illustrer le fonctionnement des méthodes itératives de manière didactique. Il fournira enfin sous une forme aussi compacte que possible des précisions sur les champs d'application de ces techniques et sur leurs qualités respectives.

2006

Chargement

Microchip-based ion analysis combining sample pretreatement, capillary electrophoresis and conductivity detection.

Université de Neuchâtel2002

Chargement

Capillary electrophoresis hyphenated to inductively coupled plasma-mass spectrometry: A novel approach for the analysis of anticancer metallodrugs in human serum and plasma

Michael Groessl, Christian Hartinger

The development of metal-based chemotherapeutics lacks methods which are capable of providing early indication on the potential of new metal complexes as future anticancer drugs. Since most of these compounds are administered intravenously, serum proteins are the first available biological binding partners in the bloodstream. For platinum-based anticancer drugs the interaction with serum proteins is regarded as an important contribution to the side effects accompanying chemotherapy. In contrast, newly developed ruthenium compounds are thought to be transported into the tumor in a protein-bound form. In here, the application of CE hyphenated to inductively coupled plasma (ICP)-MS, applying Polybrene-coated capillaries, is demonstrated for studying the interaction of indazolium [trans-tetrachlorobis(1H-indazole)ruthenate(III)] (KP1019) with HSA and transferrin, which are important transport proteins. Furthermore, the applicability of the method to human serum and plasma and, more importantly, to real-world patient samples was proven. KP1019 was found to bind to a high degree to HSA both in serum, plasma and the patient samples. Only minor fractions of ruthenium were found attached to other proteins.

2008

Chargement