Concept

Hybridation in situ

Publications associées (59)

Diminished hippocalcin expression in Huntington's disease brain does not account for increased striatal neuron vulnerability as assessed in primary neurons

Ozgün Gökce, Nikita Rudinskiy, Nicole Déglon

Hippocalcin is a neuronal calcium sensor protein previously implicated in regulating neuronal viability and plasticity. Hippocalcin is the most highly expressed neuronal calcium sensor in the medium spiny striatal output neurons that degenerate selectively ...

Wiley-Blackwell2009

GDNF-secreting human neural progenitor cells increase tyrosine hydroxylase and VMAT2 expression in MPTP-treated cynomolgus monkeys

Patrick Aebischer

Human neural progenitor cells (hNPCs) have been proposed as a potential source of cells for ex vivo gene therapy. In this pilot study, three 5-year-old female cynomolgus monkeys received a single intracarotid infusion of MPTP, followed 1 week later by MRI- ...

2008

Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection

Florian Maria Wurm, David Hacker, Madiha Derouazi, Sébastien Chenuet, Nicolas Jaccard

Transfection with polyethylenimine (PEI) was evaluated as a method for the generation of recombinant Chinese hamster ovary (CHO DG44) cell lines by direct comparison with calcium phosphate-DNA coprecipitation (CaPO4) using both green fluorescent protein (G ...

2008

Experimental comparison between conventional and hybrid long-range surface plasmon waveguide bends

Olivier Martin, Claudio Dellagiacoma

We report on the characterization of long-range surface plasmon waveguide bends at telecom wavelengths (lambda=1550 nm). The structures consist of a thin Au stripe embedded in a transparent polymer film. When the polymer thickness is larger than the latera ...

2008

Integration of a microfluidic flow cell on a CMOS biosensor for DNA detection

Nico de Rooij, Peter Van der Wal

This paper describes the fabrication technique for the realization a microfluidic flow cell to be integrated on a CMOS biosensor for DNA hybridization detection. The main element of the microfluidic system is made in polydimethylsiloxane (PDMS) elastomer a ...

IEEE Service Center, 445 Hoes Lane, Po Box 1331, Piscataway, Nj 08855-1331 Usa2008

A fully electronic label-free DNA sensor chip

Sandro Carrara, Carlotta Guiducci

This paper presents a microfabricated DNA chip for fully electronic, label-free DNA recognition, based on capacitance measurements. The chip has been fabricated in 0.5-mu m CMOS technology and it features an array of individually addressable sensing sites ...

2007

Interface Layering Phenomena in Capacitance Detection of DNA with Biochips

Giovanni De Micheli, Sandro Carrara, Carlotta Guiducci, Yusuf Leblebici, Luca Benini, Bruno Samori

Reliable DNA detection is of great importance for the development of the Lab-on-chip technology. The effort of the most recent projects on this field is to integrate all necessary operations, such as sample preparation (mixing, PCR amplification) together ...

2007

Tissue collection for systematic phenotyping in the mouse

Johan Auwerx

In this unit, a procedure for post-mortem examination of mice and tissue collection is provided. This procedure is performed for post-mortem analysis of anatomical defects (necropsy) and histological analysis and/or tissue collection destined for molecular ...

Wiley2007

Semiconductor oxide based electrodes for the label-free electrical detection of DNA hybridization: Comparison between Sb doped SnO2 and CdIn2O4

Carlotta Guiducci

First results are reported regarding the design, fabrication and operation of a DNA biochip based on a semiconductor oxide electrode that employs label-free electrical detection of the DNA hybridization. The same process of DNA functionalisation, including ...

2006

Heterogeneous oligonucleotide-hybridization assay based on hot electron-induced eletrochemiluminescence of a rhodamine label at oxide-coated aluminium and silicon electrodes

Nico de Rooij

This paper describes a heterogeneous oligonucleotide-hybridization assay based on hot electron-induced electrochemiluminescence (HECL) of a rhodamine label. Thin oxide-film coated aluminum and silicon electrodes were modified with an aminosilane layer and ...

2006

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