Concept

Hybridation in situ

Publications associées (59)

Aerobic granular sludge reactor technology Microbial diversity of aerobic granules

• Goal The goal of the project was to produce biomolecular fingerprints in order to describe the structure of microbial community which develops during aerobic sludge granulation, initiated in sequencing batch wastewater treatment processes. Four SBR were ...

2006

Genetic characterization of CHO production host DG44 and derivative recombinant cell lines

Florian Maria Wurm, David Hacker, Madiha Derouazi, Martin Bertschinger

The dihydrofolate reductase-deficient Chinese hamster ovary (CHO) cell line DG44 is the dominant mammalian host for recombinant protein manufacturing, in large part because of the availability of a well-characterized genetic selection and amplification sys ...

2006

Development and functionalisation of Sb doped SnO2 thin films for DNA biochip applications

Carlotta Guiducci, Olivier Renault

In view of developing DNA biochips based on electric detection, well controlled DNA functionalisation of conductive substrate electrode is a challenging process. In this study, the whole process of functionalisation onto home made conductive films with con ...

2006

Metallic oxide CdIn2O4 films for the label free electrochemical detection of DNA hybridization

Carlotta Guiducci

DNA functionalised semiconductor metallic oxide electrodes have been developed for the direct electrochemical detection of DNA hybridization, without labelling or the introduction of a redox couple. Conductive CdIn2O4 thin films with controlled properties ...

2006

Hardware-software design of a smart sensor for fully-electronic DNA hybridization detection

Carlotta Guiducci

This paper describes the design of a smart sensor for label-free detection of DNA hybridization. The sensor is based on a direct electrical transduction principle: it measures impedance variation at the interface between a bio-functionalized electrode and ...

2005

The quenching of electrochemiluminescence upon oligonucleotide hybridization

Nico de Rooij

Many genomic assays rely on a distance-dependent interaction between luminescent labels, such as luminescence quenching or resonance energy transfer. We studied the interaction between electrochemically excited Ru(bpy) 3 2+ and Cy5 in a hybridization assay ...

2004

DNA detection by integrable electronics

Carlotta Guiducci, Giampaolo Zuccheri

This paper presents a new electronic methodology to detect DNA hybridization for rapid identification of diseases, as well as food and environmental monitoring on a genetic base. The proposed solution exploits a new (electrical) capacitive measurement circ ...

2004

Absolute mRNA concentrations from sequence-specific calibration of oligonucleotide arrays

Felix Naef

Oligonucleotide microarrays are based on the hybridization of labeled mRNA molecules to short length oligonucleotide probes on a glass surface. Two effects have been shown to affect the raw data: the sequence dependence of the probe hybridization propertie ...

Oxford University Press2003

Reversible immobilization of peptides: Surface modification and in situ detection by attenuated total reflection FTIR spectroscopy

Horst Vogel, Patrik Willi Hoffmann, Per Rigler, Michael Mayer

A generic method is described for the reversible immobilization of polyhistidine-bearing polypeptides and proteins on attenuated total reflecting (ATR) sensor surfaces for the detection of biomol. interactions by FTIR spectroscopy. Nitrilotriacetic acid (N ...

2003

Liver receptor homolog 1 controls the expression of carboxyl ester lipase

Johan Auwerx, Kristina Schoonjans

The orphan nuclear receptor liver receptor homolog 1 (LRH-1) plays a central role in cholesterol homeostasis by regulating a number of hepatic and intestinal genes critical for reverse cholesterol transport and bile acid homeostasis. Herein, we describe th ...

2003

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