Publications associées à ADN polymérase

Spatial confinement induces hairpins in nicked circular DNA

Giovanni Dietler, Aleksandre Japaridze, Enzo Orlandini

In living cells, DNA is highly confined in space with the help of condensing agents, DNA binding proteins and high levels of supercoiling. Due to challenges associated with experimentally studying DNA under confinement, little is known about the impact of ...

Oxford University Press (OUP)2017

Cdk2 strengthens the intra-S checkpoint and counteracts cell cycle exit induced by DNA damage

Gérald Joseph Paul Lossaint, Katarina Bacevic

Although cyclin-dependent kinase 2 (Cdk2) controls the G1/S transition and promotes DNA replication, it is dispensable for cell cycle progression due to redundancy with Cdk1. Yet Cdk2 also has non-redundant functions that can be revealed in certain genetic ...

Springer Nature2017

SMiLE-seq identifies binding motifs of single and dimeric transcription factors

Didier Trono, Bart Deplancke, Giovanna Ambrosini, Philipp Bucher, Riccardo Dainese, Daniel Alpern, Michaël Imbeault, Romain Fernand Pietro Groux, Alina Isakova, Pernille Yde Rainer

Resolving the DNA-binding specificities of transcription factors (TFs) is of critical value for understanding gene regulation. Here, we present a novel, semiautomated protein-DNA interaction characterization technology, selective microfluidics-based ligand ...

Nature Publishing Group2017

Geometrical Effect in 2D Nanopores

Aleksandra Radenovic, Andras Kis, Duncan Thomas Lindsay Alexander, Vasiliki Tileli, Dumitru Dumcenco, Martina Lihter, Ke Liu, Davide Deiana, Aditya Sarathy

A long-standing problem in the application of solid-state nanopores is the lack of the precise control over the geometry of artificially formed pores compared to the well-defined geometry in their biological counterpart, that is, protein nanopores. To date ...

American Chemical Society2017

Cooperative motion of a key positively charged residue and metal ions for DNA replication catalyzed by human DNA Polymerase-eta

Matteo Dal Peraro, Vito Genna

Trans-lesion synthesis polymerases, like DNA Polymerase-eta (Pol-eta), are essential for cell survival. Pol-eta bypasses ultraviolet-induced DNA damages via a two-metal-ion mechanism that assures DNA strand elongation, with formation of the leaving group p ...

Oxford University Press (OUP)2016

Single Molecule Localization and Discrimination of DNA–Protein Complexes by Controlled Translocation Through Nanocapillaries

Aleksandra Radenovic, Sebastian Maerkl, Sanjin Marion, Roman Bulushev, Sebastian James Davis, Ekaterina Emilova Petrova

Through the use of optical tweezers we performed controlled translocations of DNA-protein complexes through nanocapillaries. We used RNA polymerase (RNAP) with two binding sites on a 7.2 kbp DNA fragment and a dCas9 protein tailored to have five binding si ...

American Chemical Society2016

Quantification of Cooperativity in Heterodimer-DNA Binding Improves the Accuracy of Binding Specificity Models

Vassily Hatzimanikatis, Bart Deplancke, Alina Isakova, Yves Berset

Many transcription factors (TFs) have the ability to cooperate on DNA elements as heterodimers. Despite the significance of TF heterodimerization for gene regulation, a quantitative understanding of cooperativity between various TF dimer partners and its i ...

American Society for Biochemistry and Molecular Biology2016

TRF2-Mediated Control of Telomere DNA Topology as a Mechanism for Chromosome-End Protection

Eric Aeby, Jing Ye, Bei Pei

The shelterin proteins protect telomeres against activation of the DNA damage checkpoints and recombinational repair. We show here that a dimer of the shelterin subunit TRF2 wraps similar to 90 bp of DNA through several lysine and arginine residues localiz ...

Cell Press2016

GreA and GreB Enhance Expression of Escherichia coil RNA Polymerase Promoters in a Reconstituted Transcription-Translation System

Sebastian Maerkl, Zoe Newell Swank, Henrike Marie Niederholtmeyer

Cell-free environments are becoming viable alternatives for implementing biological networks in synthetic biology. The reconstituted cell-free expression system (PURE) allows characterization of genetic networks under defined conditions but its applicabili ...

Amer Chemical Soc2016

Chattez avec Graph Search

Posez n’importe quelle question sur les cours, conférences, exercices, recherches, actualités, etc. de l’EPFL ou essayez les exemples de questions ci-dessous.

AVERTISSEMENT : Le chatbot Graph n'est pas programmé pour fournir des réponses explicites ou catégoriques à vos questions. Il transforme plutôt vos questions en demandes API qui sont distribuées aux différents services informatiques officiellement administrés par l'EPFL. Son but est uniquement de collecter et de recommander des références pertinentes à des contenus que vous pouvez explorer pour vous aider à répondre à vos questions.

ADN polymérase

Spatial confinement induces hairpins in nicked circular DNA

Cdk2 strengthens the intra-S checkpoint and counteracts cell cycle exit induced by DNA damage

SMiLE-seq identifies binding motifs of single and dimeric transcription factors

Geometrical Effect in 2D Nanopores

Cooperative motion of a key positively charged residue and metal ions for DNA replication catalyzed by human DNA Polymerase-eta

Single Molecule Localization and Discrimination of DNA–Protein Complexes by Controlled Translocation Through Nanocapillaries

Quantification of Cooperativity in Heterodimer-DNA Binding Improves the Accuracy of Binding Specificity Models

TRF2-Mediated Control of Telomere DNA Topology as a Mechanism for Chromosome-End Protection

Regulating the anticancer properties of organometallic dendrimers using pyridylferrocene entities: synthesis, cytotoxicity and DNA binding studies

GreA and GreB Enhance Expression of Escherichia coil RNA Polymerase Promoters in a Reconstituted Transcription-Translation System

Graph Chatbot

Chattez avec Graph Search

Single Molecule Localization and Discrimination of DNA–Protein Complexes by Controlled Translocation Through Nanocapillaries

SMiLE-seq identifies binding motifs of single and dimeric transcription factors

Quantification of Cooperativity in Heterodimer-DNA Binding Improves the Accuracy of Binding Specificity Models

Regulating the anticancer properties of organometallic dendrimers using pyridylferrocene entities: synthesis, cytotoxicity and DNA binding studies

GreA and GreB Enhance Expression of Escherichia coil RNA Polymerase Promoters in a Reconstituted Transcription-Translation System

Spatial confinement induces hairpins in nicked circular DNA

Cooperative motion of a key positively charged residue and metal ions for DNA replication catalyzed by human DNA Polymerase-eta

TRF2-Mediated Control of Telomere DNA Topology as a Mechanism for Chromosome-End Protection

Cdk2 strengthens the intra-S checkpoint and counteracts cell cycle exit induced by DNA damage

Geometrical Effect in 2D Nanopores