Concept

Quenching

Publications associées (134)

Spectral and kinetic fluorescence properties of native and nonisomerizing retinal in bacteriorhodopsin

Majed Chergui, Stefan Haacke, Selma Schenkl

Steady-state and picosecond (ps) fluorescence studies of wild-type bacteriorhodopsin (wt-bR) and of a nonisomerizing analog locked in the all-trans configuration have been performed. Extending earlier work done by femtosecond absorption spectroscopy, we ob ...

2001

Mapping the Antagonist Binding Site of the Serotonin Type 3 Receptor by Fluorescence Resonance Energy Transfer

Horst Vogel, Rudolf Hovius, Horst Pick

We have measured fluorescence resonance energy transfer (FRET) between a fluorescent antagonist, bound to the purified detergent-solubilized serotonin type 3 receptor, and a lipophilic acceptor probe partitioned into the micelle surrounding the detergent-s ...

2001

Time-resolved autofluorescence spectroscopy of the bronchial mucosa for the detection of early cancer: Clinical results

Hubert van den Bergh, Georges Wagnières, Tanja Gabrecht, Pascal Uehlinger

Time-resolved measurements of endogenous tissue autofluorescence were carried out on the bronchial mucosa of 18 patients during endoscopy by the means of an optical fibre-based spectrometer. The objective was to assess the fluorescence lifetime as a new co ...

2001

Absolute autofluorescence spectra of human healthy, metaplastic, and early cancerous bronchial tissue in vivo

Hubert van den Bergh, Georges Wagnières, Matthieu Philippe Emmanuel Zellweger

Autofluorescence is emerging as a useful tool for the detection of early cancers in the bronchi. It has already produced interesting results, which have been implemented in commercial imaging devices. Their design relies on the spectroscopy of the tissues ...

2001

Time-Resolved Autofluorescence Spectroscopy of the Bronchial Mucosa for the Detection of Early Cancer: Ex vivo and Clinical Results

Hubert van den Bergh, Georges Wagnières, Tanja Gabrecht, Pascal Uehlinger

Time-resolved measurements of endogenous tissue autofluorescence were carried out on the bronchial mucosa of 18 patients during endoscopy by the means of a optical fibre-based spectrometer. The objective was to assess the fluorescence lifetime as a new con ...

2001

Fluorescence Resonance Energy Transfer Shows a Close Helix-Helix Distance in the Transmembrane M13 Procoat Protein

Horst Vogel, Serge Cattarinussi

During the membrane insertion process the major coat protein of bacteriophage M13 assumes a conformation in which two transmembrane helixes corresponding to the leader sequence and the anchor region in the mature part of the protein coming into close conta ...

2001

Towards the fluorescence resonance energy transfer (FRET) scanning near-field optical microscopy: Investigation of nanolocal FRET processes and FRET probe microscope

Majed Chergui, Giovanni Dietler

The fluorescence resonance energy-transfer (FRET) process was studied between donor dye mols. deposited on the sample surface and acceptor dye mols. deposited on the tips of scanning near-field and at. force microscopes. The FRET process was obsd. only whe ...

2000

Absorption and fluorescence spectra of Ar-matrix-isolated Ag3 clusters

Wolfgang Harbich, Christoph Sieber

The absorption and fluorescence spectra of silver trimers isolated in an Ar matrix show the existence of three distinct lattice sites whereby the population of each site depends on the conditions of matrix preparation. Irradiation of certain absorption ban ...

2000

Matrix isolation spectroscopy of C70 - vibrational analysis and assignment of the lowest excited states

Majed Chergui

Highly structured fluorescence and fluorescence excitation spectra of C70 in Ne matrixes are reported. Fluorescence from both S1 and S2 states is obsd., and these are identified as being of A2' and E1' and have energies of 15,524 cm-1 and 15,691 cm-1, resp ...

1999

Investigation of nanolocal fluorescence resonance energy transfer for scanning probe microscopy

Majed Chergui, Giovanni Dietler

Fluorescence resonance energy transfer (FRET) was obsd. between donor dye mols. deposited onto the sample surface and acceptor dye mols. deposited onto the scanning near-field optical microscope (SNOM) or at. force microscope tip. FRET was obsd. only when ...

1999

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