Concept

Quenching

Publications associées (134)

Probing ligand-receptor recognition in G protein-coupled receptors through biosynthetic incorporation of fluorescent amino acids at specific sites and measurement of distances by fluorescence energy transfer

A review with 6 refs. of the authors' work in developing a biophys. technique to investigate the structure, function and dynamics of membrane receptors. A fluorescence-based approach has been developed and applied to the prototypic G protein-coupled recept ...

1998

Mapping the binding sites of peptide and non-peptide molecules to G protein-coupled receptors by fluorescence

Gerardo Turcatti

Novel fluorescence approaches to investigate ligand recognition and structure of G protein-coupled receptors in native membranes have been developed. These methods combine the biosynthetic incorporation of unnatural fluorescent amino acids at known sites i ...

1998

Characterization of non-peptide antagonist and peptide agonist binding sites of the NK1 receptor with fluorescent ligands

Gerardo Turcatti

Ligand recognition of the NK1 receptor (substance P receptor) by peptide agonist and non-peptide antagonist has been investigated and compared by the use of fluorescent ligands and spectrofluorometric methods. Analogues of substance P (SP) labeled with the ...

1997

Development of an optical fiber fluorescence setup for in-situ PAHs detection in porous media. Application to pyranine transport in sand columns

Hubert van den Bergh, André Mermoud

An optical method based on fluorescence spectroscopy was developed for in-situ non-destructive, real time, org. pollutant detection and quantification in soil. Optical fiber-based light-induced fluorescence probes allowing in-situ specific chem. detection ...

1997

Fluorescent labeling of NK2 receptor at specific sites in vivo and fluorescence energy transfer analysis of NK2 ligand-receptor complexes

Horst Vogel, Gerardo Turcatti, Jonathan Knowles

A fluorescent unnatural amino acid was introduced biosynthetically at known sites into the G protein-coupled neurokinin (tachykinin) NK2 receptor by suppression of UAG nonsense codons with the aid of a chem. misacylated synthetic tRNA specifically designed ...

1997

An endoscopic fluorescence imaging system for simultaneous visual examination and photodetection of cancers

Hubert van den Bergh, Georges Wagnières, André Studzinski

We describe the design and performance tested during six years of clinical trials of a fluorescence endoscope for the detection and delineation of cancers in several hollow organs. The apparatus is based on the imaging of the laser-induced fluorescence tha ...

1997

Clinical imaging fluorescence apparatus for the endoscopic photodetection of early cancers by use of Photofrin II

Hubert van den Bergh, Christian Depeursinge, Georges Wagnières, André Châtelain, André Studzinski

A fluorescence imaging device applied to the detection of early cancer is described. The app. is based on the imaging of laser-induced fluorescence of a dye that localizes in a tumor with a higher concn. than in the surrounding normal tissue after i.v. inj ...

Optical Society of America1997

Clinical imaging fluorescence apparatus for the endoscopic photodetection of early cancers by use of Photofrin II

Christian Depeursinge, Georges Wagnières, André Studzinski

A fluorescence imaging device applied to the detection of early cancer is described. The apparatus is based on the imaging of laser-induced fluorescence of a dye that localizes in a tumor with a higher concentration than in the surrounding normal tissue af ...

1997

Metal ion trace detection by a chelator-modified gold electrode: A comparison of surface to bulk affinity

Horst Vogel, Rudolf Hovius, Thierry Stora

The binding of metal ions to self-assembled nitrilotriacetic acid (NTA)-modified thioalkane monolayers was monitored by impedance spectroscopy via a capacitance change of the blocking gold electrode. Binding to a fluorescent NTA-derivative and the native N ...

American Chemical Society1997

Optical microscopy in the nano-world

Olivier Martin, Abdeljalil Sayah, Mirjam Pfeiffer, Marc Jobin

Scanning near-field optical microscopy (SNOM) is an optical microscopy whose resolution is not bound to the diffraction limit. It provides chemical information based upon spectral, polarization and/or fluorescence contrast images. Details as small as 20 nm ...

1997

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