Concept

Selectable marker

Publications associées (31)

Scalable Reverse Engineering of Nonlinear Gene Networks

Dario Floreano, Claudio Mattiussi, Daniel Marbach, Thomas Schaffter

Current advanced molecular biology techniques provide gene expression (mRNA) levels of selected genes of an organism. In such aggregate, the mRNA level of one gene is mediated by the presence of specific proteins and metabolites produced by other genes. Ge ...

2008

Analysis of potential transcriptomic biomarkers for Huntington's disease in peripheral blood

Mauro Delorenzi, Heike Runne

Highly quantitative biomarkers of neurodegenerative disease remain an important need in the urgent quest for disease-modifying therapies. For Huntington's disease (HD), a genetic test is available (trait marker), but necessary state markers are still in de ...

National Academy of Sciences2007

Notch signaling is required for normal prostatic epithelial cell proliferation and differentiation

Freddy Radtke, Michel Aguet, Zhipeng Tang

Notch pathway is crucial for stem/progenitor cell maintenance, growth and differentiation in a variety of tissues. Using a transgenic cell ablation approach, we found in our previous study that cells expressing Notch1 are crucial for prostate early develop ...

2006

Serum-free large-scale transient transfection of CHO cells

Florian Maria Wurm, Madiha Derouazi, Natalie Muller, Martin Bertschinger, Philippe Pierre Girard

To date, methods for large-scale transient gene expression (TGE) in cultivated mammalian cells have focused on two transfection vehicles: polyethylenimine (PEI) and calcium phosphate (CaPi). Both have been shown to result in high transfection efficiencies ...

2004

Directed Evolution of O6-Alkylguanine-DNA Alkyltransferase for Efficient Labeling of Fusion Proteins with Small Molecules In Vivo

Horst Vogel, Kai Johnsson, Horst Pick, Alexandre Juillerat, Thomas Gronemeyer, Antje Keppler

We report here the generation of mutants of the human O6-alkylguanine-DNA alkyltransferase (hAGT) for the efficient in vivo labeling of fusion proteins with synthetic reporter mols. Libraries of hAGT were displayed on phage, and mutants capable of efficien ...

2003

Targeting exogenous genes to tumor angiogenesis by transplantation of genetically modified hematopoietic stem cells

Michele De Palma

Angiogenic tumor vessels are promising targets for the activity and the selective delivery of cancer therapeutics. The bone marrow contributes different cell types to the tumor stroma, including hematopoietic cells and, as recently suggested, vascular endo ...

Nature Publishing Group2003

Surface grafting onto template-assembled synthetic protein scaffolds in molecular recognition

Patricia Durieux

A review with 35 refs. Creating functional biol. mols. de novo requires a detailed understanding of the intimate relationship between primary sequence, folding mechanism, and packing topol., and remains up to now a most challenging goal in protein design a ...

2001

The mouse Hoxd13(spdh) mutation, a polyalanine expansion similar to human type II synpolydactyly (SPD), disrupts the function but not the expression of other Hoxd genes

Denis Duboule

Polyalanine expansion in the human HOXD13 gene induces synpolydactyly (SPD), an inherited congenital limb malformation. A mouse model was isolated, which showed a spontaneous alanine expansion due to a 21-bp duplication at the corresponding place in the mo ...

2001

Directed molecular evolution of cytochrome c peroxidase

Kai Johnsson, Petra Tafelmeyer

Cytochrome c peroxidase (CCP) from Saccharomyces cerevisiae was subjected to directed mol. evolution to generate mutants with increased activity against the classical peroxidase substrate, guaiacol, thus changing the substrate specificity of CCP from the p ...

2000

Phage display of combinatorial peptide and protein libraries and their applications in biology and chemistry

Kai Johnsson

A review with 100 refs. The enormous potential of combinatorial approaches for studying problems in biol. and chem. has been clearly demonstrated over the last several years. In particular, phage display has become one of the major techniques for the use o ...

1999

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