Publications associées à Cell counting

Portable impedance-sensing device for microorganism characterization in the field

A variety of biosensors have been proposed to quickly detect and measure the properties of individual microorganisms among heterogeneous populations, but challenges related to cost, portability, stability, sensitivity, and power consumption limit their app ...

Nature Portfolio2023

Counting using deep learning regression gives value to ecological surveys

Devis Tuia, Benjamin Alexander Kellenberger

Many ecological studies rely on count data and involve manual counting of objects of interest, which is time-consuming and especially disadvantageous when time in the field or lab is limited. However, an increasing number of works uses digital imagery, whi ...

Nature Research2021

Impedance-Based Single-Cell Pipetting

Philippe Renaud, Yann Barrandon, David Vincent Bonzon, Jean-Baptiste Bureau, Georges Muller, Nicolas Jean Bernard Beuchat, Nicolas Uffer

Many biological methods are based on single-cell isolation. In single-cell line development, the gold standard involves the dilution of cells by means of a pipet. This process is time-consuming as it is repeated over several weeks to ensure clonality. Here ...

SAGE PUBLICATIONS INC2020

How to improve the sensitivity of coplanar electrodes and micro channel design in electrical impedance flow cytometry: a study

Philippe Renaud, Jonathan Bastien Cottet, Harald Van Lintel

This paper describes a comprehensive analysis of the geometrical parameters in uencing the sensitivity of a coplanar elec- trode layout for electrical impedance ow cytometry. The designs presented in this work have been simulated, fabricated, and tested. 3 ...

2019

Impedance Spectroscopy for Single Cell Analysis and Dispensing

David Vincent Bonzon

Flow cytometry is an essential tool in biology and medicine with applications in immunodeficiency diagnostic, immunosurveillence, organ transplantation in addition to its basic research usage. Before the widespread use of the Fluorescent-Activated Cell Sor ...

EPFL2017

Microfluidic-integrated vertical electrodes employed in impedance-based cytometry : potential application in immunotherapies

Enrica Rollo

During the last decades, the growing interest for single-cell analysis has led to the creation of a number of microfluidic and lab-on-a chip (LOC) platforms for characterizing cellular samples. In that context label-free based platforms are minimally invas ...

EPFL2016

An impedance-based flow microcytometer for single cell morphology discrimination

Philippe Renaud, Ludovica Colella, Marjan Shaker

Cell shape is a fundamental biological feature, providing specific information about physiological or pathological cellular conditions. Most of the state-of-the-art microfluidic cytometers, however, only allow simple cell analysis, including viability stud ...

Royal Soc Chemistry2014

Detecting particles flowing through interdigitated 3D microelectrodes

Carlotta Guiducci, Enrico Accastelli, Samuel Kilchenmann, Elena Bianchi, Enrica Rollo

Counting cells in a large microchannel remains challenging and is particularly critical for in vitro assays, such as cell adhesion assays. This paper addresses this issue, by presenting the development of interdigitated three-dimensional electrodes, which ...

Ieee2012

Cell viability assessment by flow cytometry using yeast as cell model

Philippe Renaud, Nina Buffi, Niccolo Piacentini, Raphaël Tornay

This paper reports the new combination of cell sorting and counting capabilities on a single device. Most state-of-the-art devices combining these technologies use optical techniques requiring complicated experimental setups and labeled samples. The use of ...

2011

Targeting of multiple metabolites in neural cells monitored by using protein-based carbon nanotubes

Giovanni De Micheli, Sandro Carrara, Cristina Boero, Giovanna Del Vecchio

Microdevices dedicated to monitor metabolite levels have recently enabled many applications in the field of cell analysis, to monitor cell growth and development of numerous cell lines. By combining the traditional technology used for electrochemical biose ...

2011