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The identification of all protein targets of a small molecule drug, i.e. target deconvolution, provides the basis for understanding its beneficial or deleterious actions. Target deconvolution remains a major and often problematic task within the biotechnol ...
For several decades, hepatotoxicity has been a major issue for drug development.Recently, three-dimensional (3D) cell culture has been believed to improve cell drug response accuracy. However important limitations such as slow production, lack of cell dens ...
Transient gene expression (TGE) is based on episomal DNA expression. This technique is used for the production of recombinant proteins that can be used as therapeutics. TGE is for example extremely useful for shortening the time between candidate proteins ...
We present a novel OptoMEA platform that combines multisite electrical recording with local chemical stimulation. Applying UV light pulses through an array of optical fibres aligned to transparent indium-tin oxide electrodes of an MEA biochip leads to loca ...
The foundations for fluorescence correlation spectroscopy (FCS) were already laid in the early 1970s, but this technique did not become widely used until single-molecule detection was established almost 20 years later with the use of diffraction-limited co ...
Cell cycle synchronization is an important tool for the study of the cell division stages and signalling. It provides homogeneous cell cultures that are of importance to develop and improve processes such as protein synthesis and drug screening. The main a ...
Recombinant protein-based biologics are increasingly relevant in the pharmaceutical industry. Currently, mammalian cell-based bioprocesses are a routine manufacturing procedure for complex proteins such as recombinant antibodies, and the number of new biol ...
Approximately 2% of amyotrophic lateral sclerosis (ALS) cases are associated with mutations in the cytosolic Cu/Zn superoxide dismutase 1 (SOD1) gene. Transgenic SOD1 mice constitute useful models of ALS to screen therapeutical approaches. Glial cell line- ...
A review. With the advances in genomics, proteomics and functional genomics new therapeutic targets to be tackled by medicinal chem. are expected. This article reviews applications of first principle methods to address medicinal chem. issue related to drug ...
A review. The efficient anal. of the function of ion channels is an important task in many different fields ranging from (bio)analytics and drug-screening to nanoelectronics. Here, the modulation of the channel activity of a synthetic ligand gated ion chan ...