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Cyclo-beta-tetrapeptide I, a beta-peptidic analog of octreotide, was prepd. from beta-amino acid building blocks synthesized by Arndt-Eistert homologation of appropriately protected alpha-amino acids. The affinity of I for five different human somatostatin ...
Reversible and oriented immobilization of proteins in a functionally active form on solid surfaces is a prerequisite for the investigation of mol. interactions by surface-sensitive techniques. We demonstrate a method generally applicable for the attachment ...
G protein-coupled receptors (GPCRs) constitute an abundant family of membrane receptors of high pharmacol. interest. Cell-based assays are the predominant means of assessing GPCR activation, but are limited by their inherent complexity. Functional mol. ass ...
We have expressed the 5-HT3 receptor with two transient expression sytems: the Semliki Forest virus and the HEK293EBNA. Both systems allow the rapid expression of functional receptor protein at very high level: up to 107 receptors per cell. Large scale pro ...
A fluorescently labeled ligand for the 5-HT3 serotonin receptor was synthesized and its sub-nanomolar affinity for the purified, detergent solubilized receptor was measured. The change in the ligand's fluorescence upon receptor binding was used to directly ...
The recently described tests of the synchrotron imaging photoelectron spectromicroscope MEPHISTO (Microscope a Emission de PHotoelectrons par Illumination Synchrotronique de Type Onduleur) were complemented by further resolution improvements and tests, whi ...
A direct immunosensor has been developed using an acoustic wave device as a transducer. The device is based on an acoustic waveguide geometry that supports a Love wave. The biorecognition surface, formed on a gold layer, consisted of a biotinylated support ...
A serotonin 5-HT3 receptor was functionally expressed to high levels and on a large scale in mammalian cells with the Semliki Forest virus system. Conditions were optimized to maximize detergent solubilization of the receptor, while preserving ligand bindi ...
Anti- V.cholerae monoclonal IgA antibodies have been produced using a hybridoma cell line (Zac3) grown in RPMI medium contg. 10% Fetal calf serum (FCS) and a completely defined protein- free medium. The latter has been optimized with respect to amino acid, ...
Material surfaces that can mediate cellular interactions by the coupling of specific cell membrane receptors may allow for the design of a biomaterial that can control cell attachment, differentiation, and tissue organization. Cell adhesion proteins have b ...
