Biomasse (écologie) | EPFL Graph Search

vignette|upright=1.5|Biomasse totale des organismes présents sur Terre vignette|upright=1.5|Biomasse totale des animaux présents sur Terre vignette|redresse=1.3|Selon les données « couleur de l'océan » recueillies par le capteur SeaWiFS, la production primaire dans l'océan mondial est sensiblement égale à celle sur les terres émergées, bien que la biomasse primaire océanique soit environ 500 fois moins importante que la biomasse terrestre, ce qui traduit la très grande efficacité du phytoplancton océanique. redresse=1.3|vignette|Carte mondiale de la distribution de chlorophylle. Les régions où la production de phytoplancton est abondante sont cependant très restreintes (franges côtières des régions tropicales, Pacifique Nord et Atlantique Nord, et océan austral), formant des « prairies océaniques ». La majeure partie des aires marines forme des « déserts océaniques », d'où la belle couleur bleue des eaux tropicales. En écologie, la biomasse est le terme qui désigne la masse totale d'

Optimization of drip irrigation using a sensor network in Sahel Region (Burkina Faso) - Analysis of soil water and plant dynamics in the framework of project Info4Dourou 2.0

Tom Müller

The research project Info4Dourou 2.0 's main goal is to improve agriculture in semi-arid regions by developing a support system that optimizes agricultural production and water consumption based on continuous soil humidity measurements using a sensor network. In this context, the main purpose of the study was to give more scientific support to the agronomic model on which the sensor network is based. In particular the research aims at understanding the soil water and plant dynamics in order to give recommendations on the system design. The main feature of the system is to indicate when irrigation must be triggered and in which soil moisture conditions. The thesis developed a numerical model based on the software HYDRUS 2D to acquire a precise knowledge on the soil water dynamics and was calibrated and coupled with field experiments on two vegetable crops of eggplant and cabbage in Burkina Faso using drip kit irrigation systems. A great focus was given on the plant response to different irrigation schedules and to water stress, considering aerial biomass development, root distribution and final yields. Water stress was linked to continuous measurements of the soil matrix potential at different depth and locations. The field experiments showed that a daily irrigation frequency resulted to better canopy development during the first part of the growth but that great water savings are possible by optimizing the schedule. Water stress was difficult to track precisely by following daily sap flow behaviour which suggested that plant stress occurs before transpiration reduction, especially when biomass is building up. The adaptability of the root distribution was demonstrated and was clearly correlated with the wetted zone which depended on the irrigation schedule. From the numerical model, it was shown that using one sensor at a depth of about 10 cm was most appropriate in order to pilot irrigation during the whole crop growth. The analysis showed that most of the water savings could be achieved at relatively high threshold values and that low thresholds mainly resulted to transpiration reduction which was not advisable. A threshold of -20 kPa was proposed for the beginning of the growth stage which then decreases to a value of -50 kPa at full canopy development. These thresholds are believed to be adequate for most vegetable crops since they allow keeping the soil matrix potential in the root zone at values that are tolerated by most plants. An analysis of the influence of the soil texture also showed that similar values seem to perform well for most soils, if assuming an adaptation of the root distribution to the wetted zone. The study focused mainly on water stress but more research could be done on the impact of the irrigation system on nutrients availability for example.

2015

Co-culture of microalgae and bacteria for wastewater treatment

Thierry Mounir

To address the problem of insucient wastewater treatment and eutrophication of lakes and rivers, the symbiotic association of microalgae and bacteria has shown great potential and numerous advantages. First, co-cultures can exhibit ecient treatment of domestic wastewater, with the combination of high nutrients (nitrogen and phosphorus) removal abilities of the microalgae and ecient degradation of organic matter by bacteria. Second, the symbiosis between microalgae and bacteria exchanging CO2 and O2 makes aeration not necessary, thus signicantly reducing the operating costs of the wastewater treatment plant. Third, recovered biomass of algae could be used as highpro t products like biofuels and food source. Fourth, the xation of the atmospheric CO2 by the microalgae through photosynthesis would contribute to greenhouse gas reduction in the atmosphere. Finally, the decayed microalgal biomass could serve as organic carbon source for denitrication by bacteria. However, this technology is not well-known yet, and further research on the in uence of the operating parameters have to be conducted. Furthermore, its main drawback is the harvesting of the microalgae. Due to their small size, microalgae demonstrate a low settling rate of 3.6 x 10􀀀3 m/h. A solution is to enhance aggregation of the biomass through occulation, followed by granulation to reach fully-developed microalgal-bacterial consortia. These granules exhibit high treatment performances and high settleability. The rst objective of this project was to identify the optimal microalgae:bacteria ratio for COD and nutrients removal. The second objective was to form fully-developed granules, starting from a co-culture, and assess the in uence of dierent agitation speeds on the granulation. The photobioreactor with a microalgae:bacteria ratio of 75:25 expressed the most promising results in COD removal eciency (95% after 8 days), nitrogen removal eciency (94% after 9 days), phosphorus removal eciency (93% after 7 days), and biomass productivity (144 mg/L/d). In this photobioreactor, nitrogen was removed by a combination of assimilation into microalgal biomass and nitrication/denitrication by the bacteria. The COD was removed by degradation by bacteria and mixotrophic microalgae that used the organic matter as carbon source. Finally, phosphorus was removed by assimilation into microalgal biomass. Although obvious aggregation between microalgae and bacteria could be observed, no fully-developed granules were formed after 89 batches of 24 hours. Because of low COD and nitrogen removals, no starvation period was experienced by the co-cultures. Therefore, extracellular polymeric substances were not released in sucient amounts to enhance the granulation. Furthermore, excessive stirring (200 rpm) may have lead to microalgal cells damage, thus promoting bacterial growth over microalgal growth. On the other hand, slower agitation speeds (80 and 120 rpm) seemed to enhance microalgal growth. Overall, the agitation speed appeared to have an indirect impact on the treatment performances and the settleability of the co-cultures, by signicantly in uencing the microalgal and bacterial growths.

2020

Wastewater and green waste biorefinery Degradation of simple sugars using mixed cultures of purple non-sulfur bacteria: an insight into microbial interactions

Guillaume Crosset-Perrotin

Purple non-sulfur bacteria (PNSB) are phototrophic organisms that have raised interest for both industrial and domestic wastewater treatment and valorization because of their very high assimilation (~1 g CODX g-1 COD substrate) and valuable compounds production (i.e hydrogen, polyhydroxyalkanoates, pigments). PNSB have been studied mostly in pure- or co-cultures with fermentative bacteria. Mixed cultures have been little described. This work aims to enrich a stable association of fermenters and PNSB on a carbohydrate-rich wastewater in a single-step process with a mixed culture. Batches with glucose and acetate at different concentrations were inoculated with an in-house mixed-culture. A chemostat was continuously supplied with glucose (5 g COD L-1) and run at a dilution rate of 0.05 h-1 after start-up under batch mode and baseline stabilization of the PNSB enrichment with acetate. The reactor was constantly illuminated with a light filtered at wavelengths superior to 700 nm. Biomass, pigments expression and substrate depletion were tracked along the experiment. A model was developed to anticipate the biomass growth and the substrates consumption. In the batches fed with acetate, PNSB were dominant in purple biomass (>80% vs. total sequencing read count). The main PNSB population were Rhodopseudomonas and Rhodobacter. With glucose, the biomass turned white. The fermenter Enterobacter dominated the microbiomes (>95% abundance). In the stirred-tank reactor, batch operation with acetate led to the production of a substantial biomass enriched in PNSB (4.0 g VSS L-1) that assimilated the nutrients in a molar C-N-P ratio of 100:9.5:0.1 (- n/n/n). Under chemostat regime with glucose, PNSB were efficiently associated with fermenters in a biomass of 1.0-1.5 g VSS L-1. Phase-contrast microscopy displayed the typical morphotypes of Rhodopseudomonas and the fermenter Clostridium. The glucose was completely depleted. The main fermentation products were acetate (between 3 and 10 mmol L-1), ethanol (between 6 and 9 mmol L-1) and butyrate (between 2 and 4 mmol L-1). The hydrogen yield was low (0.033±0.012 mol H2 mol-1 glucose). A fermenters-PNSB mixed culture was successfully built by microbial community engineering by harnessing the chemostat regime to establish the ecological niches and interactions of these organisms. It provides an excellent opportunity to clean carbohydrate-rich wastewater in a compact single-stage process. In the case of direct application of PNSB, a concentrated biomass can be produced. The biomass can even find interesting applications for resource recovery in the form of, e.g., a protein-rich fertilizing biomass (on food and agricultural aqueous wastes), biogas, bulk chemicals and biopolymers.

2019