Concept

Culture microbienne

Publications associées (146)

Sequentially Modified, Polymer-Stabilized Gold Nanoparticle Libraries: Convergent Synthesis and Aggregation Behavior

Harm-Anton Klok, Matthew Gibson, Maarten Danial

This manuscript describes a versatile, yet experimentally facile, method for producing libraries of polymer coated (core-shell type) gold nanoparticles. The synthetic principle relies on two, sequential postmodification reactions, which ensures homogeneity ...

2011

Mycobacterium tuberculosis persistence mutants identified by screening in isoniazid-treated mice

John McKinney, Neeraj Dhar

Tuberculosis (TB) is notoriously difficult to cure, requiring administration of multiple antibiotics for 6 mo or longer. Conventional anti-TB drugs inhibit biosynthetic processes involved in cell growth and division, such as DNA replication, RNA transcript ...

2010

A multichamber fluidic device for 3D cultures under interstitial flow with live imaging: Development, characterization, and applications

Melody Swartz, Jan Overney, James Brandon Dixon, Adrian Chung-Kai Shieh, Carmen Bonvin

Interstitial flow is an important biophysical cue that can affect capillary morphogenesis, tumor cell migration, and fibroblast remodeling of the extracellular matrix, among others. Current models that incorporate interstitial flow and that are suitable fo ...

Wiley-Blackwell2010

Sigma Factor F does not Prevent Rifampin Inhibition of RNA Polymerase or Cause Rifampin Tolerance in Mycobacterium tuberculosis

Stewart Cole, Florence Pojer, Claudia Sala, Ruben Hartkoorn, Jeffrey Chen, Sophie Magnet

The tolerance of Mycobacterium tuberculosis to anti-tuberculosis drugs is a major reason for the lengthy therapy needed to treat a tuberculosis infection. Rifampin is a potent inhibitor of RNA polymerase (RNAP) in vivo, but has been shown to be less effect ...

2010

Carbon Nanotubes-Based Electrochemical Sensing for Cell Culture Monitoring

Giovanni De Micheli, Sandro Carrara, Cristina Boero, Giovanna Del Vecchio

Monitoring of metabolic compounds, such as glucose and lactate, is extensively reported in literature, especially for clinical purposes. Instead, the application of such technologies for monitoring metabolites in cell cultures has not been explored. From o ...

2010

Biological and structural characterization of the Mycobacterium smegmatis nitroreductase NfnB, and its role in benzothiazinone resistance

Stewart Cole, Joao Pedro De Almeida Neres, Giulia Manina

Summary Tuberculosis is still a leading cause of death in developing countries, for which there is an urgent need for new pharmacological agents. The synthesis of the novel antimycobacterial drug class of benzothiazinones (BTZs) and the identification of t ...

2010

Selective Breast Cancer Cell Capture, Culture, and Immunocytochemical Analysis Using Self-Assembled Magnetic Bead Patterns in a Microfluidic Chip

Martinus Gijs, Jean-Claude Bünzli, Caroline Vandevyver, Venkataragavalu Sivagnanam, Bo Song

Separation and subsequent culturing of MCF-7 breast cancer cells on self-assembled protein-coated magnetic beads in a microfluidic chip is demonstrated. The beads were patterned in situ inside a sealed microfluidic channel using magnetic-field-assisted ele ...

American Chemical Society2010

New Approaches towards Organic Photodetection and Bio-Integration

Diego Ghezzi

We demonstrate hybrid, solid-liquid photodiodes, using semiconducting conjugated polymers as active materials and ionic liquids as unconventional cathodes (water, saline solutions, physiological buffers, cell-culturing media). We give a complete opto-elect ...

2010

Biomaterials: Spotlight on hydrogels

Matthias Lütolf

By using light to control the degradation of hydrogel components in space and time, researchers have generated a tool to help them reconstruct functional biological tissues in a culture dish. ...

2009

Separation of antibiotic-treated mycobacterial subpopulations using multiple-frequency dielectrophoresis

Philippe Renaud, John McKinney, Thomas Michael Braschler, Ana Valero

Exposure of a bacterial culture to an antibiotic or other lethal stress typically results in the generation of sub-populations of live cells ("persisters") and dead cells, and, in some cases, viable but non-culturable cells. Studies aimed at understanding ...

2009

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