Concept
Antagoniste (biochimie)
Publications associées (134)
Study of ligand-receptor interactions by fluorescence correlation spectroscopy with different fluorophores: Evidence that the homopentameric 5-hydroxytryptamine type 3(As) receptor binds only one ligand
Horst Vogel, Rudolf Hovius, Kirstin Friedrich
The 5-hydroxytryptamine receptor of type 3 was investigated by fluorescence correlation spectroscopy (FCS). Binding constants of fluorescently labeled ligands, the stoichiometry, and the mass of the receptor are readily accessible by this technique, while ...
1999The subtype 2 of angiotensin II receptors and pressure-natriuresis in adult rat kidneys
1 The present work examined the effects of the subtype 2 of angiotensin II (AT(2)) receptors on the pressure-natriuresis using a new peptide agonist, and the possible involvement of cyclic guanosine 3', 5' monophosphate (cyclic GMP) in these effects. 2 In ...
1999Fluorescence techniques for fundamental and applied studies of membrane protein receptors: the 5-HT3 serotonin receptor
A fluorescently labeled ligand for the 5-HT3 serotonin receptor was synthesized and its sub-nanomolar affinity for the purified, detergent solubilized receptor was measured. The change in the ligand's fluorescence upon receptor binding was used to directly ...
1999Transient-expression technologies, their application and scale-up: 5-HT3 serotonin receptor case study
We have expressed the 5-HT3 receptor with two transient expression sytems: the Semliki Forest virus and the HEK293EBNA. Both systems allow the rapid expression of functional receptor protein at very high level: up to 107 receptors per cell. Large scale pro ...
PORTLAND PRESS1999