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Today, more than 30 products using a manufacturing process based on mammalian cell culture have been approved for human therapy. Most of these products are currently supplied with stirred tank bioreactors operated in batch or fed-batch mode. However, the b ...
An agitated 12-well microtiter plate system with a working volume of 2ml was investigated for cell culture process development. Agitation assures homogeneity in wells and enhances mass transfer between the gas and the liquid phase, thus improving maximum c ...
A unique bench-scale heat flux calorimeter is described, which is capable of detecting the low heat signal of an animal cell culture (a max. of 200 mW/l is produced with a batch culture of SF9 insect cells). After the tech. modifications that enhanced the ...
Bench-scale biocalorimetry (>=1 L) allows for the detn. of the metabolic heat flow during bioprocesses under complete control of all process conditions for extended periods of time. It can be combined with a no. of online and off-line measurement technique ...
The expression of recombinant proteins with the Semliki Forest Virus (SFV) system has been scaled up to bioreactor scale. As a model protein for this study the human 5-HT(3) receptor was chosen. The gene for the receptor was subcloned into the SFV expressi ...
Systematic errors due to the neglect of water and/or ethanol partition between liq. and gaseous phases are discussed for bioreactors equipped with or without a condenser. Both water vapor and ethanol vapor are present in the off-gas leaving the condenser. ...
Mid-IR (MIR) spectroscopy in combination with partial least-squares (PLS) regression anal. was used to monitor the concns. of glucose, lactate, ammonia, and asparagine in situ during bioreactor cultivation of CHO/SSF3 cells. Simple PLS calibration models w ...
A review with 29 refs. of lab.-scale investigations carried out on the usefulness of biol. heat-release measurements, as a means for monitoring and controlling the metabolic state of microbial cultures. Such studies are carried out in high-quality bench-sc ...
Segmented flow comprising discrete volumes of a liquid reaction mixture separated by discrete volumes of immiscible fluid is passed through a tubular reactor for residence times sufficient to precipitate a powder product from the reaction mixture. USE-Manu ...
The transient behavior of the dehydration of isopropanol has been studied in a microreactor. The av. productivity under periodic operation is found to be considerably higher compared to steady state even for conversions reaching 80%. The optimal cycle peri ...
