Microbial population biology is the application of the principles of population biology to microorganisms. Microbial population biology, in practice, is the application of population ecology and population genetics toward understanding the ecology and evolution of bacteria, archaebacteria, microscopic fungi (such as yeasts), additional microscopic eukaryotes (e.g., "protozoa" and algae), and viruses. Microbial population biology also encompasses the evolution and ecology of community interactions (community ecology) between microorganisms, including microbial coevolution and predator-prey interactions. In addition, microbial population biology considers microbial interactions with more macroscopic organisms (e.g., host-parasite interactions), though strictly this should be more from the perspective of the microscopic rather than the macroscopic organism. A good deal of microbial population biology may be described also as microbial evolutionary ecology. On the other hand, typically microbial population biologists (unlike microbial ecologists) are less concerned with questions of the role of microorganisms in ecosystem ecology, which is the study of nutrient cycling and energy movement between biotic as well as abiotic components of ecosystems. Microbial population biology can include aspects of molecular evolution or phylogenetics. Strictly, however, these emphases should be employed toward understanding issues of microbial evolution and ecology rather than as a means of understanding more universal truths applicable to both microscopic and macroscopic organisms. The microorganisms in such endeavors consequently should be recognized as organisms rather than simply as molecular or evolutionary reductionist model systems. Thus, the study of RNA in vitro evolution is not microbial population biology and nor is the in silico generation of phylogenies of otherwise non-microbial sequences, even if aspects of either may in some (especially unintentional) manner be analogous to evolution in actual microbial populations.

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